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- W2243314104 abstract "Article1 August 1987free access Structural and transcription analysis of two homologous genes for the P700 chlorophyll a-apoproteins in Chlamydomonas reinhardii: evidence for in vivo trans-splicing Ulrich Kück Ulrich Kück Lehrstuhl für Allgemeine Botanik, Ruhr-Universität Bochum, Postfach 10 21 48, D-4630 Bochum 1, FRG Search for more papers by this author Yves Choquet Yves Choquet Institut de Biologie Physico-Chimique, 13, Rue Pierre et Marie Curie, 75005 Paris Search for more papers by this author Michel Schneider Michel Schneider Institut de Biologie Physico-Chimique, 13, Rue Pierre et Marie Curie, 75005 Paris Search for more papers by this author Michel Dron Michel Dron Universite de Paris-Sud, Centre d'Orsay, Biologie Moléculaire Végétale, Bâtiment 430, 91405 Orsay Cedex, France Search for more papers by this author Pierre Bennoun Pierre Bennoun Institut de Biologie Physico-Chimique, 13, Rue Pierre et Marie Curie, 75005 Paris Search for more papers by this author Ulrich Kück Ulrich Kück Lehrstuhl für Allgemeine Botanik, Ruhr-Universität Bochum, Postfach 10 21 48, D-4630 Bochum 1, FRG Search for more papers by this author Yves Choquet Yves Choquet Institut de Biologie Physico-Chimique, 13, Rue Pierre et Marie Curie, 75005 Paris Search for more papers by this author Michel Schneider Michel Schneider Institut de Biologie Physico-Chimique, 13, Rue Pierre et Marie Curie, 75005 Paris Search for more papers by this author Michel Dron Michel Dron Universite de Paris-Sud, Centre d'Orsay, Biologie Moléculaire Végétale, Bâtiment 430, 91405 Orsay Cedex, France Search for more papers by this author Pierre Bennoun Pierre Bennoun Institut de Biologie Physico-Chimique, 13, Rue Pierre et Marie Curie, 75005 Paris Search for more papers by this author Author Information Ulrich Kück1, Yves Choquet2, Michel Schneider2, Michel Dron3 and Pierre Bennoun2 1Lehrstuhl für Allgemeine Botanik, Ruhr-Universität Bochum, Postfach 10 21 48, D-4630 Bochum 1, FRG 2Institut de Biologie Physico-Chimique, 13, Rue Pierre et Marie Curie, 75005 Paris 3Universite de Paris-Sud, Centre d'Orsay, Biologie Moléculaire Végétale, Bâtiment 430, 91405 Orsay Cedex, France The EMBO Journal (1987)6:2185-2195https://doi.org/10.1002/j.1460-2075.1987.tb02489.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info The two homologous genes for the P700 chlorophyll a-apoproteins (ps1A1 and ps1A2) are encoded by the plastom in the green alga Chlamydomonas reinhardii. The structure and organization of the two genes were determined by comparison with the homologous genes from maize using data from heterologous hybridizations as well as from DNA and RNA sequencing. While the ps1A2 (736 codons) gene shows a continuous gene organization, the ps1A1 (754 codons) gene possesses some unusual features. The discontinuous gene is split into three separate exons which are scattered around the circular chloroplast genome. Exon 1 (86 bp) is separated by ∼50 kb from exon 2 (198 bp), which is located ∼ 90 kb apart from exon 3 (1984 bp). All exons are flanked by intronic sequences of group II. Transcription analysis reveals that the ps1A2 gene hybridizes with a 2.8-kb transcript, while all exon regions of the ps1A1 gene are homologous to a mature mRNA of 2.7 kb. From our data we conclude that the three distantly separated exonic sequences of the ps1A1 gene constitute a functional gene which probably operates by a trans-splicing mechanism. Previous ArticleNext Article Volume 6Issue 81 August 1987In this issue RelatedDetailsLoading ..." @default.
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- W2243314104 title "Structural and transcription analysis of two homologous genes for the P700 chlorophyll <i>a</i> -apoproteins in <i>Chlamydomonas reinhardii</i> : evidence for <i>in vivo trans</i> -splicing" @default.
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