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- W2243356165 abstract "The clinical applications of tissue engineering are still limited by the lack of a functional vascular supply in tissue-engineered constructs. In order to improve the pre-vascularization of tissue-engineered scaffold during in vitro culture, in this study, based on three-dimensional (3D) printing technology, using the crosslinking effect of coaxial fluids (sodium alginate and CaCl 2 ) to prepare vessel-like hollow gel fibers, then layer by layer overlapping into 3D scaffold. The biological 3D printing platform was successfully developed and a coaxial nozzle module was introduced to generate a CaCl 2 -in-Alginate coaxial microfluidic. The inner core diameters of the prepared hollow gel fibers were 220~380 micrometers. In addition, the influence of materials concentration and dispensing rates on hollow fiber dimension were investigated, the cell-encapsulated in the printed hollow fibers was realized and the viability of endothelial cells (ECs) was studied with Laser scanning confocal microscopy (LSCM) and Live-Dead cell staining. The 3D scaffold built by hollow fibers could improve the phenomenon of diffusion constrain and enhance the survival rate of those ECs growing at a greater depth in the construct. This study provides a new theoretical basis for the vascularization of bone scaffold." @default.
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- W2243356165 date "2015-05-01" @default.
- W2243356165 modified "2023-09-27" @default.
- W2243356165 title "A Biological 3D Printer for the Preparation of Tissue Engineering Micro-Channel Scaffold" @default.
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- W2243356165 doi "https://doi.org/10.4028/www.scientific.net/kem.645-646.1290" @default.
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