Matches in SemOpenAlex for { <https://semopenalex.org/work/W2243357811> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W2243357811 abstract "Abstract Objective The aim of this work was to evaluate the role of ubiquitin–proteasome system (UPS) on mitochondrial-driven alpha-synuclein (aSN) clearance in in vitro, ex vivo and in vivo Parkinson's disease (PD) cellular models. Method We used SH-SY5Y ndufa2 knock-down (KD) cells, PD cybrids and peripheral blood mononuclear cells (PBMC) from patients meeting the diagnostic criteria for PD. We quantified aSN aggregation, proteasome activity and protein ubiquitination levels. In PBMC of PD patient population we evaluated the aSN levels in the plasma and the influence of several demographic characteristics in the above mentioned determinations. Results We found that ubiquitin-independent proteasome activity was up-regulated in SH-SY5Y ndufa2 KD cells while a downregulation was observed in PD cybrids and PBMC. Moreover, we observed an increase in protein ubiquitination that correlates with a decrease in ubiquitin-dependent proteasome activity. Accordingly, proteasome inhibition prevented ubiquitin-dependent aSN clearance. Ubiquitin-independent proteasome activity was positively correlated with ubiquitination in PBMC. We also report a negative correlation of chymotrypsin-like activity with age in control and late-onset PD groups. Total ubiquitin content is positively correlated with aSN oligomer levels, which leads to an age-dependent increase of aSN ubiquitination in LOPD. Moreover, aSN levels are increased in the plasma of PD patients. Interpretation aSN oligomers are ubiquitinated and we identified a ubiquitin-dependent clearance insufficiency with the accumulation of both aSN and ubiquitin. However, SH-SY5Y ndufa2 KD cells showed a significant up-regulation of ubiquitin-independent proteasomal enzymatic activity that could mean a cell rescue attempt. Moreover, we identified that UPS function is age-dependent in PBMC." @default.
- W2243357811 created "2016-06-24" @default.
- W2243357811 creator A5002113898 @default.
- W2243357811 creator A5025517129 @default.
- W2243357811 creator A5045379471 @default.
- W2243357811 creator A5047194377 @default.
- W2243357811 creator A5049145557 @default.
- W2243357811 creator A5057579955 @default.
- W2243357811 creator A5060915659 @default.
- W2243357811 creator A5074146037 @default.
- W2243357811 date "2012-08-29" @default.
- W2243357811 modified "2023-09-27" @default.
- W2243357811 title "Ubiquitin proteasome system in Parkinson's Disease: a keeper or a witness?" @default.
- W2243357811 doi "https://doi.org/10.7490/f1000research.1092129.1" @default.
- W2243357811 hasPublicationYear "2012" @default.
- W2243357811 type Work @default.
- W2243357811 sameAs 2243357811 @default.
- W2243357811 citedByCount "0" @default.
- W2243357811 crossrefType "journal-article" @default.
- W2243357811 hasAuthorship W2243357811A5002113898 @default.
- W2243357811 hasAuthorship W2243357811A5025517129 @default.
- W2243357811 hasAuthorship W2243357811A5045379471 @default.
- W2243357811 hasAuthorship W2243357811A5047194377 @default.
- W2243357811 hasAuthorship W2243357811A5049145557 @default.
- W2243357811 hasAuthorship W2243357811A5057579955 @default.
- W2243357811 hasAuthorship W2243357811A5060915659 @default.
- W2243357811 hasAuthorship W2243357811A5074146037 @default.
- W2243357811 hasConcept C104317684 @default.
- W2243357811 hasConcept C127561419 @default.
- W2243357811 hasConcept C137061746 @default.
- W2243357811 hasConcept C185592680 @default.
- W2243357811 hasConcept C202751555 @default.
- W2243357811 hasConcept C207001950 @default.
- W2243357811 hasConcept C25602115 @default.
- W2243357811 hasConcept C27740335 @default.
- W2243357811 hasConcept C54355233 @default.
- W2243357811 hasConcept C55493867 @default.
- W2243357811 hasConcept C71924100 @default.
- W2243357811 hasConcept C86803240 @default.
- W2243357811 hasConcept C95444343 @default.
- W2243357811 hasConceptScore W2243357811C104317684 @default.
- W2243357811 hasConceptScore W2243357811C127561419 @default.
- W2243357811 hasConceptScore W2243357811C137061746 @default.
- W2243357811 hasConceptScore W2243357811C185592680 @default.
- W2243357811 hasConceptScore W2243357811C202751555 @default.
- W2243357811 hasConceptScore W2243357811C207001950 @default.
- W2243357811 hasConceptScore W2243357811C25602115 @default.
- W2243357811 hasConceptScore W2243357811C27740335 @default.
- W2243357811 hasConceptScore W2243357811C54355233 @default.
- W2243357811 hasConceptScore W2243357811C55493867 @default.
- W2243357811 hasConceptScore W2243357811C71924100 @default.
- W2243357811 hasConceptScore W2243357811C86803240 @default.
- W2243357811 hasConceptScore W2243357811C95444343 @default.
- W2243357811 hasLocation W22433578111 @default.
- W2243357811 hasOpenAccess W2243357811 @default.
- W2243357811 hasPrimaryLocation W22433578111 @default.
- W2243357811 hasRelatedWork W1984618096 @default.
- W2243357811 hasRelatedWork W1993138670 @default.
- W2243357811 hasRelatedWork W1993561057 @default.
- W2243357811 hasRelatedWork W2018228226 @default.
- W2243357811 hasRelatedWork W2022287604 @default.
- W2243357811 hasRelatedWork W2034716576 @default.
- W2243357811 hasRelatedWork W2057358353 @default.
- W2243357811 hasRelatedWork W2060059654 @default.
- W2243357811 hasRelatedWork W2071995179 @default.
- W2243357811 hasRelatedWork W2074026845 @default.
- W2243357811 hasRelatedWork W2086457894 @default.
- W2243357811 hasRelatedWork W2089707823 @default.
- W2243357811 hasRelatedWork W2089748347 @default.
- W2243357811 hasRelatedWork W2101335451 @default.
- W2243357811 hasRelatedWork W2114939802 @default.
- W2243357811 hasRelatedWork W2117890091 @default.
- W2243357811 hasRelatedWork W2161016587 @default.
- W2243357811 hasRelatedWork W2508266305 @default.
- W2243357811 hasRelatedWork W2845825737 @default.
- W2243357811 hasRelatedWork W3007256515 @default.
- W2243357811 hasVolume "3" @default.
- W2243357811 isParatext "false" @default.
- W2243357811 isRetracted "false" @default.
- W2243357811 magId "2243357811" @default.
- W2243357811 workType "article" @default.