Matches in SemOpenAlex for { <https://semopenalex.org/work/W2244086702> ?p ?o ?g. }
Showing items 1 to 61 of
61
with 100 items per page.
- W2244086702 abstract "Plasmodium species (the causative agents of malaria), and related parasites of the phylum Apicomplexa such as Toxoplasma gondii, have a plastid organelle of endosymbiotic origin. This organelle is regarded as a potential new drug target. It contains its own genome encoding many of the proteins and RNAs required for protein synthesis, but its function is unclear. Nucleus-encoded proteins are likely to control the primary function and biogenesis of the plastid and I have studied two of these: FtsZ (involved in bacterial and plastid division), and Ycfl6 (a protein of unknown function that may interact with Ycf24, encoded on the plastid genome). First I set up a screen to check that any genes I identified were malarial in origin, and not from the mycoplasma that contaminate our cultures. Searches for ftsZ by degenerate PCR, database analysis and low-stringency hybridisation, were unsuccessful. The possibility remains that the apicomplexan plastid divides by some other mechanism, as mitochondria and some bacteria do, for example using a dynamin-related protein. A dynamin-like sequence was identified on chromosome 14 in P. falciparum, and its transcription at the appropriate stage of the erythrocytic cycle was confirmed. However, this protein could be involved in mitochondrial division. AycfI6-like sequence was found on chromosome 14 of P. falciparum and expressed in Escherichia coli for antibody production. This sequence encodes a putative plastid-targeting amino-terminal peptide; reporter protein and immunofluorescence studies will confirm whether it is localised in the plastid. To study the function of ycfl6,1 disrupted an orthologous version in the cyanobacterium Synechocystis and found it is an essential gene whose partial loss was deleterious. The δycfl6 mutant cyanobacteria were arrested in cytokinesis and resembled those found previously in our laboratory for δycf24 mutants, but the effects were less severe. I confirmed that ycf24 and ycfl6 are co-transcribed in Synechocystis, and perhaps form part of the same operon. These findings add weight to the hypothesis that Ycfl6 and Ycf24 act in the same pathway, and that the imported Ycfl6 and the endogenous plastid protein Ycf24 are both essential for plastid maintenance. Based on recent additions to the literature, I propose that Ycfl6 is involved in maturation of Fe-S cluster-containing proteins required for resistance to oxidative stress and the provision of reducing power for biosynthetic pathways that occur in the plastid of apicomplexans." @default.
- W2244086702 created "2016-06-24" @default.
- W2244086702 creator A5086239148 @default.
- W2244086702 date "2001-01-01" @default.
- W2244086702 modified "2023-09-27" @default.
- W2244086702 title "Nuclear genes involved in maintenance of the malarial plastid" @default.
- W2244086702 hasPublicationYear "2001" @default.
- W2244086702 type Work @default.
- W2244086702 sameAs 2244086702 @default.
- W2244086702 citedByCount "0" @default.
- W2244086702 crossrefType "dissertation" @default.
- W2244086702 hasAuthorship W2244086702A5086239148 @default.
- W2244086702 hasConcept C104317684 @default.
- W2244086702 hasConcept C131934819 @default.
- W2244086702 hasConcept C141231307 @default.
- W2244086702 hasConcept C2775855988 @default.
- W2244086702 hasConcept C2776237524 @default.
- W2244086702 hasConcept C54355233 @default.
- W2244086702 hasConcept C547475151 @default.
- W2244086702 hasConcept C69305403 @default.
- W2244086702 hasConcept C86803240 @default.
- W2244086702 hasConcept C93829228 @default.
- W2244086702 hasConcept C95444343 @default.
- W2244086702 hasConceptScore W2244086702C104317684 @default.
- W2244086702 hasConceptScore W2244086702C131934819 @default.
- W2244086702 hasConceptScore W2244086702C141231307 @default.
- W2244086702 hasConceptScore W2244086702C2775855988 @default.
- W2244086702 hasConceptScore W2244086702C2776237524 @default.
- W2244086702 hasConceptScore W2244086702C54355233 @default.
- W2244086702 hasConceptScore W2244086702C547475151 @default.
- W2244086702 hasConceptScore W2244086702C69305403 @default.
- W2244086702 hasConceptScore W2244086702C86803240 @default.
- W2244086702 hasConceptScore W2244086702C93829228 @default.
- W2244086702 hasConceptScore W2244086702C95444343 @default.
- W2244086702 hasLocation W22440867021 @default.
- W2244086702 hasOpenAccess W2244086702 @default.
- W2244086702 hasPrimaryLocation W22440867021 @default.
- W2244086702 hasRelatedWork W116455179 @default.
- W2244086702 hasRelatedWork W126702956 @default.
- W2244086702 hasRelatedWork W1966666429 @default.
- W2244086702 hasRelatedWork W2010616193 @default.
- W2244086702 hasRelatedWork W2038914716 @default.
- W2244086702 hasRelatedWork W2071546827 @default.
- W2244086702 hasRelatedWork W2106535769 @default.
- W2244086702 hasRelatedWork W2109352225 @default.
- W2244086702 hasRelatedWork W2123398236 @default.
- W2244086702 hasRelatedWork W2132990510 @default.
- W2244086702 hasRelatedWork W2133308481 @default.
- W2244086702 hasRelatedWork W2142857824 @default.
- W2244086702 hasRelatedWork W2150767846 @default.
- W2244086702 hasRelatedWork W2157257368 @default.
- W2244086702 hasRelatedWork W224655620 @default.
- W2244086702 hasRelatedWork W27586646 @default.
- W2244086702 hasRelatedWork W2856423467 @default.
- W2244086702 hasRelatedWork W3109524960 @default.
- W2244086702 hasRelatedWork W50446145 @default.
- W2244086702 hasRelatedWork W71634682 @default.
- W2244086702 isParatext "false" @default.
- W2244086702 isRetracted "false" @default.
- W2244086702 magId "2244086702" @default.
- W2244086702 workType "dissertation" @default.