Matches in SemOpenAlex for { <https://semopenalex.org/work/W2244290654> ?p ?o ?g. }
- W2244290654 abstract "Currently, the U.S. market for propionate is about 400 million pounds per year, and the demand is increasing at 3 ~ 4 % annually. Fermentation process can use byproducts from the food industry, such as corn steep liquor and cheese whey, to produce propionic acid. It is thus desirable to convert these low-grade byproducts to a marketable product, such as propionic acid by fermentation. However, propionic acid fermentation with Propionibacterium acidipropionici is limited by the low product yield due to its heterofermetation nature. Metabolic engineering to modify the dicarboxylic acid pathway can be an effective method to alter carbon flow to improve propionate yield by knocking-out the acetate formation. Acetate kinase (ACK) is a key enzyme involving acetate formation in the pathway, and was the knockout target in this research. Integrational mutagenesis by homologous recombination can be used to disrupt ack gene by first constructing a non-replicative plasmid with partial ack and then transforming it into cells. Integration of the non-replicative plasmid can result in duplicate repeats of the homologous regions flanking DNA sequences, and thus cause mutagenesis. In this work, partial ack, encoding ACK, was first cloned and sequenced by PCR based cloning. A 477 bp of partial ack sequence was obtained. However, low yield of genomic DNA preparation from P. acidipropionici might have attributed to failure to clone the ack gene from the genomic DNA using the degenerate" @default.
- W2244290654 created "2016-06-24" @default.
- W2244290654 creator A5067299257 @default.
- W2244290654 date "2004-01-01" @default.
- W2244290654 modified "2023-09-27" @default.
- W2244290654 title "Polymerase chain reaction based cloning of acetate kinase in propionibacterium acidipropionici" @default.
- W2244290654 cites W106745433 @default.
- W2244290654 cites W1146646623 @default.
- W2244290654 cites W1480119301 @default.
- W2244290654 cites W1483144986 @default.
- W2244290654 cites W1496782251 @default.
- W2244290654 cites W1500170643 @default.
- W2244290654 cites W1500647294 @default.
- W2244290654 cites W1502029927 @default.
- W2244290654 cites W1502555174 @default.
- W2244290654 cites W1504967140 @default.
- W2244290654 cites W1511497967 @default.
- W2244290654 cites W1524462753 @default.
- W2244290654 cites W1536831415 @default.
- W2244290654 cites W1539054325 @default.
- W2244290654 cites W1558751910 @default.
- W2244290654 cites W1561431337 @default.
- W2244290654 cites W1562424017 @default.
- W2244290654 cites W1566514223 @default.
- W2244290654 cites W1591844107 @default.
- W2244290654 cites W1592926208 @default.
- W2244290654 cites W1599073788 @default.
- W2244290654 cites W1625346333 @default.
- W2244290654 cites W1642722232 @default.
- W2244290654 cites W1670230410 @default.
- W2244290654 cites W1706688458 @default.
- W2244290654 cites W1801859858 @default.
- W2244290654 cites W1899082983 @default.
- W2244290654 cites W1925553755 @default.
- W2244290654 cites W1942690669 @default.
- W2244290654 cites W1962277393 @default.
- W2244290654 cites W1964987246 @default.
- W2244290654 cites W1969001635 @default.
- W2244290654 cites W1975906762 @default.
- W2244290654 cites W1977187453 @default.
- W2244290654 cites W1979262511 @default.
- W2244290654 cites W1984088954 @default.
- W2244290654 cites W1987804798 @default.
- W2244290654 cites W1988754502 @default.
- W2244290654 cites W1992498577 @default.
- W2244290654 cites W1993704260 @default.
- W2244290654 cites W1995724867 @default.
- W2244290654 cites W1997012629 @default.
- W2244290654 cites W2005175892 @default.
- W2244290654 cites W2008153516 @default.
- W2244290654 cites W2009188183 @default.
- W2244290654 cites W2011899079 @default.
- W2244290654 cites W2011946542 @default.
- W2244290654 cites W2013595179 @default.
- W2244290654 cites W2016844373 @default.
- W2244290654 cites W2017108581 @default.
- W2244290654 cites W2017960107 @default.
- W2244290654 cites W2020876515 @default.
- W2244290654 cites W2026139009 @default.
- W2244290654 cites W2034402092 @default.
- W2244290654 cites W2035686996 @default.
- W2244290654 cites W2036363678 @default.
- W2244290654 cites W2036376904 @default.
- W2244290654 cites W2040626944 @default.
- W2244290654 cites W2064885655 @default.
- W2244290654 cites W2073033511 @default.
- W2244290654 cites W2099108215 @default.
- W2244290654 cites W2107693255 @default.
- W2244290654 cites W2109276669 @default.
- W2244290654 cites W2119210884 @default.
- W2244290654 cites W2128694293 @default.
- W2244290654 cites W2132189624 @default.
- W2244290654 cites W2133747186 @default.
- W2244290654 cites W2136501741 @default.
- W2244290654 cites W2139749499 @default.
- W2244290654 cites W2140552895 @default.
- W2244290654 cites W2141533388 @default.
- W2244290654 cites W2142716675 @default.
- W2244290654 cites W2153639362 @default.
- W2244290654 cites W2160138916 @default.
- W2244290654 cites W2472327279 @default.
- W2244290654 cites W2487108377 @default.
- W2244290654 cites W2558192324 @default.
- W2244290654 cites W2558489444 @default.
- W2244290654 cites W2615640575 @default.
- W2244290654 cites W2616832882 @default.
- W2244290654 cites W2900940636 @default.
- W2244290654 cites W2914468275 @default.
- W2244290654 cites W3217699138 @default.
- W2244290654 cites W54046181 @default.
- W2244290654 cites W874759507 @default.
- W2244290654 cites W950006870 @default.
- W2244290654 cites W98792810 @default.
- W2244290654 hasPublicationYear "2004" @default.
- W2244290654 type Work @default.
- W2244290654 sameAs 2244290654 @default.
- W2244290654 citedByCount "0" @default.
- W2244290654 crossrefType "journal-article" @default.
- W2244290654 hasAuthorship W2244290654A5067299257 @default.
- W2244290654 hasConcept C100544194 @default.