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- W2244333473 abstract "Differentiation and identification of Erwinia species was done with specific PCR primers mainly from house keeping genes and EPS encoding regions. Within a species, strains can be distinguished by single nucleotide polymorphisms (SNP) of many genes with non-matching bases and modified conditions for PCR. Classification of isolates was done by nucleotide sequence analysis and alignments of 16S rRNA and of house keeping genes. A novel and fast method for detection and differentiation has been developed by resolution of protein patterns applying whole cells to MALDI TOF analysis. Measured in the size range of 2,000 to 20,000 Da, the detected proteins indicate the species and classify unknown strains within the genus Erwinia. The resulting dendrograms are closely related to those from nucleotide analysis. The method is useful for mass screening and identifies unknown samples by scores of protein relatedness." @default.
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- W2244333473 date "2009-01-01" @default.
- W2244333473 modified "2023-09-27" @default.
- W2244333473 title "Detection and differentiation of antagonistic Erwinia species by PCR and MALDI TOF analyses." @default.
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