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• W2244536938 abstract "The nucleocapsid protein (NC) plays an important role in retroviral replication, in part, by facilitating numerous nucleic acid rearrangements throughout the reverse transcription process. The nucleic acid chaperone activity of the human immunodeficiency virus type-1 (HIV-1) NC has been extensively studied, and duplex destabilization, nucleic acid aggregation, and rapid protein binding kinetics have been identified as major components of the activity of this highly basic protein (pI ~10). The chaperone activity of other NC proteins is not well understood. We used single molecule DNA stretching to characterize the activity of HIV-1, RSV, and MMLV NC. We found distinct differences in the chaperone activities of each protein, which reflect the requirements for nucleic acid chaperone activity in each retroviral replication system. HTLV-1 NC exhibited overall poor nucleic acid chaperone acitivity. This result is explained by its poor aggregating activity and slow dissociation from single-stranded DNA. This NC protein is overall neutral at pH=7.5 and possesses a unique, acidic C-terminal domain. By studying different HTLV-1 NC mutants, the role of C-terminal domains to the chaperone activity was elucidated. The results suggest that the electrostatic interaction between HTLV-1 NC and nucleic acids is the major factor determining the kinetics. We also examine the nucleic acid interaction properties of the Apolipoprotein B mRNA editing enzyme, a catalytic polypeptide-like 3G (APOBEC3G/A3G) that is known to inhibit HIV-1 reverse transcription in absence of viral infectivity factor (Vif). Our stretching experiments suggested a novel mechanism for deaminase-independent inhibition of reverse transcription due to vital differences of nucleic acid binding kinetics between NC, A3G and reverse transcriptase (RT). Finally, Long interspersed nucleic elements (LINE) are highly repeated nucleic acids sequences in mammal genomes. Our single DNA molecule stretching experiments characterized the nucleic acid chaperone function of ORF1p in the mouse LINE-1 retrotransposon. We found that a single amino acid substitution altered retrotransposition efficiency by a factor of 15 due to a reduction in nucleic acid chaperone activity exhibited by ORF1p. For all of the studies presented here, we used single molecule methods to characterize the nucleic acid interactions of proteins involved in reverse transcription in retroviruses or retrotransposons. In each case, complementary bulk experiments were done by collaborators. The results are presented together in each chapter of the thesis." @default.
• W2244536938 created "2016-06-24" @default.
• W2244536938 creator A5086529957 @default.
• W2244536938 date "2021-05-10" @default.
• W2244536938 modified "2023-10-17" @default.
• W2244536938 title "Single molecule force spectroscopy studies of DNA binding and chaperone proteins" @default.
• W2244536938 cites W105318823 @default.
• W2244536938 cites W1480928537 @default.
• W2244536938 cites W1489145497 @default.
• W2244536938 cites W1495862348 @default.
• W2244536938 cites W1503573373 @default.
• W2244536938 cites W1507156025 @default.
• W2244536938 cites W1515306118 @default.
• W2244536938 cites W1542093498 @default.
• W2244536938 cites W1572589552 @default.
• W2244536938 cites W1585397042 @default.
• W2244536938 cites W1602204726 @default.
• W2244536938 cites W1616088578 @default.
• W2244536938 cites W1623198081 @default.
• W2244536938 cites W1659719610 @default.
• W2244536938 cites W166408772 @default.
• W2244536938 cites W1701611052 @default.
• W2244536938 cites W1744129015 @default.
• W2244536938 cites W1796915314 @default.
• W2244536938 cites W1806634181 @default.
• W2244536938 cites W1901653080 @default.
• W2244536938 cites W1918197787 @default.
• W2244536938 cites W1963830843 @default.
• W2244536938 cites W1966343819 @default.
• W2244536938 cites W1966477735 @default.
• W2244536938 cites W1967178181 @default.
• W2244536938 cites W1967287923 @default.
• W2244536938 cites W1970054449 @default.
• W2244536938 cites W1972739934 @default.
• W2244536938 cites W1974781990 @default.
• W2244536938 cites W1976011820 @default.
• W2244536938 cites W1977422953 @default.
• W2244536938 cites W1978696046 @default.
• W2244536938 cites W1979467978 @default.
• W2244536938 cites W1981742364 @default.
• W2244536938 cites W1981932058 @default.
• W2244536938 cites W1982406314 @default.
• W2244536938 cites W1982809791 @default.
• W2244536938 cites W1984772767 @default.
• W2244536938 cites W1985309304 @default.
• W2244536938 cites W1986665516 @default.
• W2244536938 cites W1987239216 @default.
• W2244536938 cites W1988416664 @default.
• W2244536938 cites W1990273984 @default.
• W2244536938 cites W1991719330 @default.
• W2244536938 cites W1993319890 @default.
• W2244536938 cites W1995636525 @default.
• W2244536938 cites W1996756349 @default.
• W2244536938 cites W1996798386 @default.
• W2244536938 cites W1997209343 @default.
• W2244536938 cites W1997873146 @default.
• W2244536938 cites W1998193423 @default.
• W2244536938 cites W1999494370 @default.
• W2244536938 cites W1999557018 @default.
• W2244536938 cites W2000284725 @default.
• W2244536938 cites W2000424360 @default.
• W2244536938 cites W2001375348 @default.
• W2244536938 cites W2002475098 @default.
• W2244536938 cites W2005361700 @default.
• W2244536938 cites W2006699168 @default.
• W2244536938 cites W2007589425 @default.
• W2244536938 cites W2009478573 @default.
• W2244536938 cites W2009846118 @default.
• W2244536938 cites W2011120754 @default.
• W2244536938 cites W2011573266 @default.
• W2244536938 cites W2013927372 @default.
• W2244536938 cites W2014067586 @default.
• W2244536938 cites W2014207390 @default.
• W2244536938 cites W2014310905 @default.
• W2244536938 cites W2016638271 @default.
• W2244536938 cites W2017414074 @default.
• W2244536938 cites W2019280437 @default.
• W2244536938 cites W2020002410 @default.
• W2244536938 cites W2020675572 @default.
• W2244536938 cites W2022573774 @default.
• W2244536938 cites W2024364777 @default.
• W2244536938 cites W2024654096 @default.
• W2244536938 cites W2029884547 @default.
• W2244536938 cites W2030315713 @default.
• W2244536938 cites W2030365976 @default.
• W2244536938 cites W2030676469 @default.
• W2244536938 cites W2033631070 @default.
• W2244536938 cites W2033871884 @default.
• W2244536938 cites W2034889455 @default.
• W2244536938 cites W2035395679 @default.
• W2244536938 cites W2036116609 @default.
• W2244536938 cites W2037217898 @default.
• W2244536938 cites W2037259592 @default.
• W2244536938 cites W2037635062 @default.
• W2244536938 cites W2038470175 @default.
• W2244536938 cites W2038492533 @default.
• W2244536938 cites W2039121168 @default.
• W2244536938 cites W2041074982 @default.
• W2244536938 cites W2041516645 @default.
• W2244536938 cites W2044473601 @default.

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