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- W2244703495 abstract "Rapid and reliable detection of harmful algae in coastal areas and shellfish farms is an important requirement of monitoring programs. Molecular technologies are rapidly improving the detection of phytoplankton and their toxins. Assays are based on the discrimination of genetic differences within different species. A commercially available PCR ELISA Dig Detection Kit was adapted for the detection of the toxic dinoflagellate Alexandrium minutum using sandwich hybridization in a microtiter plate. A set of two probes for the species-specific identification was developed for A. minutum. The specificity of the probes was successfully demonstrated with the microtiter plate assay. A standard calibration curve for different RNA concentrations and thus cell counts was determined for the assay. Total rRNA was isolated from three different strains of A. minutum and the mean concentration of RNA per cell of was determined to be 0.028 ng. The assay and the standard curve were evaluated by using spiked water samples. The results demonstrate that the molecular assay was able to detect A. minutum cells at different cell counts in the presence of a complex background. The experiments with spiked natural samples present a proof of principle of this assay. These tests also provided the necessary specificity tests prior to the probes being adapted to an automated biosensor using a sandwich hybridization format (Metfies et al. 2005)." @default.
- W2244703495 created "2016-06-24" @default.
- W2244703495 creator A5047409501 @default.
- W2244703495 date "2007-01-01" @default.
- W2244703495 modified "2023-09-27" @default.
- W2244703495 title "Development and evaluation of rapid and semi-automated devices for the detection of toxic algae" @default.
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