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- W2244748968 abstract "BK channels, which are comprised of pore forming α and accessory β-subunits, have been shown to eliminate intracellular K in response to hypo-osmotic swelling. However, the role of the β subunits in this process has not been determined. We used HEK293 cells stably expressing BK-α and examined a role for transiently expressed BK-β4 (90% expression rate) to modulate the response of BK to cell swelling. Using patch clamp analysis, we determined NPo (cell attached, -Vp = −40 mV) and the % of observed channels per patch. The NPo and % channels per patch of BK-α expressed alone in cells in isotonic (290 mOsm) solution was 0.71±0.05 (n=3) and 53%, respectively. This value was not significantly different from the NPo and % channels per patch for BK-α in hypotonic (200 mOsm) solution (0.62±0.17, n=3 and 41%, respectively). The NPo and % channels per patch of BK-α plus BK–β4 was significantly greater in hypotonic solution (1.32±0.15, n=3 and 76%, respectively) than in isotonic solution (0.70±0.15, n=8 and 41%, respectively). Compared to isotonic solution, when cells expressing BK-α/β4 were in hypotonic solution, BK-α exhibited more intense immuno-staining at the plasma membrane and BK-β4 was increased at the plasma membrane (western blot; OD = 0.20 in isotonic and 0.49 in hypotonic). These data suggest that the BK-β4 subunit is necessary for trafficking of BK-α and BK-β4 to the plasma membrane in response to hypo-osmotic stress." @default.
- W2244748968 created "2016-06-24" @default.
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- W2244748968 date "2008-03-01" @default.
- W2244748968 modified "2023-09-28" @default.
- W2244748968 title "Role of BK‐β4 in hypo‐osmotic activation of large conductance, Ca‐sensitive K (BK) channels expressed in HEK293 cells." @default.
- W2244748968 doi "https://doi.org/10.1096/fasebj.22.1_supplement.1158.28" @default.
- W2244748968 hasPublicationYear "2008" @default.
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