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- W2245562270 abstract "Objective: To construct and prepare the recombinant adeno-associated virus (AAV) vector for expressing short hairpin RNA (shRNA) targeting Notch-1 mRNA. Methods: The Notch-1 template DNA sequence was designed, and the recombinant vector of plasmid pAAV-MCS2/siRNA-Notch-1 was constructed. The recombinant adenovirus vector AAV-siRNA-Notch-1 was obtained by homologous recombination with pAAV-MCS2/siRNA-Notch-1, pAAV-RC and pHelper in bacteria QBI-293A cells. Results: pAAV-MCS2/siRNA-Notch-1 was successfully constructed, and the rAAV with a titer of 1.2×1012 (VP/L)was obtained. Conclusion: The viral vector was successfully constructed, and it may be used in investigating the Notch-1 function in tumor stem cell." @default.
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- W2245562270 date "2010-01-01" @default.
- W2245562270 modified "2023-09-23" @default.
- W2245562270 title "Construction of recombinant adeno-associated virus vector of siRNA with targeting Notch-1." @default.
- W2245562270 hasPublicationYear "2010" @default.
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