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- W2245586125 abstract "686 Background: The tissue microarray (TMA) technique allows to assemble multiple tumor samples in the same paraffin block to evaluate multiple markers using immunohistochemistry. Methods: we have constructed TMAs of 293 breast carcinomas with complete follow-up (median 132 mos). Four tumor cores per sample (i.e.: 4 replica) were included in TMA blocks. We evaluated the expression of ER, PgR, Her2/neu (Herceptest), p53, MIB1, Bcl2, p27, p63, HMW cytokeratin (HMWCK), and FITH. The 4 different replica of each case were separately analysed by two pathologists. Her2/neu was evaluated according to FDA score. For all other markers we evaluated cellular compartimentalization, intensity (from 0 to 3) and percentage of reacting cells. Results: from the original 1172 cores in the TMAs, 78% were evaluable for each marker because of lack of cells or loss of cores during the TMA sections processing. The most relevant prognostic markers were Her2/neu and p53: they retained prognostic value even after adjusting for N st..." @default.
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- W2245586125 date "2005-06-01" @default.
- W2245586125 modified "2023-09-27" @default.
- W2245586125 title "Multiple marker analysis in breast tumors with long term follow-up using Tissue Microarray: Immunohistochemical evaluation of ER, PgR, MIB1, p27, p63, HMWCK, FITH, Her2/neu and p53" @default.
- W2245586125 doi "https://doi.org/10.1200/jco.2005.23.16_suppl.686" @default.
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