Matches in SemOpenAlex for { <https://semopenalex.org/work/W22459633> ?p ?o ?g. }
Showing items 1 to 83 of
83
with 100 items per page.
- W22459633 endingPage "43" @default.
- W22459633 startingPage "436" @default.
- W22459633 abstract "Natural infection of research mice with enterohepatic Helicobacter spp. is common and may confound experimental studies from intercurrent disease. We evaluated a protocol of dirty bedding exposure for transmission of Helicobacter infection from colony mice to female Tac:(SW)fBR sentinel mice over 6 months.Cecal scrapings from culled colony mice and associated sentinel mice were tested for H. hepaticus, H. rodentium, and H. bilis using polymerase chain reaction analysis (PCR). These results were correlated with the results of sentinel serum IgG responses measured by ELISA.In 9 colony rooms, 43 of 45 mice were infected with H. hepaticus; in 14 rooms, 58 of 70 mice were infected with H. rodentium; and in 2 rooms, 2 of 10 mice were infected with H. bilis. Concurrence of Helicobacter infection between colony and sentinel mice was 82% for H. hepaticus, 88% for H. rodentium, and 94% for H. bilis. Concurrence of Helicobacter infection status of sentinel cagemates was 98% for H. hepaticus, 86% for H. rodentium, and 95% for H. bilis. Fecal samples pooled by sentinel cage had positive PCR results for H. hepaticus and H. rodentium at 1 month in 60 and 44%, respectively, of the cages that contained test-positive mice at necropsy (6 months). By 3 months, detection rates were 100 and 81% for H. hepaticus and H. rodentium, respectively, and H. bilis was not detected until 4 months. Newly acquired infections with H. rodentium and H. bilis were evident throughout the 6-month study period. Seroconversion was coincident with positive PCR results in sentinel mice, and serum IgG values continued to increase until necropsy. The serum IgG ELISA was 98 to 100% sensitive, but was low in specificity (34 to 44%), most likely attributable to common coinfection with H. hepaticus and H. rodentium.Sentinel mice acquire infection with Helicobacter spp. through dirty bedding exposure. Combined use of PCR analysis and serologic testing of sentinel mice was predictive of Helicobacter infection status of mouse colonies used for biomedical research." @default.
- W22459633 created "2016-06-24" @default.
- W22459633 creator A5006632183 @default.
- W22459633 creator A5011449507 @default.
- W22459633 creator A5024428264 @default.
- W22459633 creator A5026189340 @default.
- W22459633 creator A5038189979 @default.
- W22459633 creator A5052028175 @default.
- W22459633 creator A5085156862 @default.
- W22459633 date "2000-08-01" @default.
- W22459633 modified "2023-09-29" @default.
- W22459633 title "Monitoring sentinel mice for Helicobacter hepaticus, H rodentium, and H bilis infection by use of polymerase chain reaction analysis and serologic testing." @default.
- W22459633 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11020164" @default.
- W22459633 hasPublicationYear "2000" @default.
- W22459633 type Work @default.
- W22459633 sameAs 22459633 @default.
- W22459633 citedByCount "31" @default.
- W22459633 countsByYear W224596332012 @default.
- W22459633 countsByYear W224596332013 @default.
- W22459633 countsByYear W224596332015 @default.
- W22459633 countsByYear W224596332016 @default.
- W22459633 countsByYear W224596332020 @default.
- W22459633 countsByYear W224596332021 @default.
- W22459633 countsByYear W224596332023 @default.
- W22459633 crossrefType "journal-article" @default.
- W22459633 hasAuthorship W22459633A5006632183 @default.
- W22459633 hasAuthorship W22459633A5011449507 @default.
- W22459633 hasAuthorship W22459633A5024428264 @default.
- W22459633 hasAuthorship W22459633A5026189340 @default.
- W22459633 hasAuthorship W22459633A5038189979 @default.
- W22459633 hasAuthorship W22459633A5052028175 @default.
- W22459633 hasAuthorship W22459633A5085156862 @default.
- W22459633 hasConcept C104317684 @default.
- W22459633 hasConcept C159047783 @default.
- W22459633 hasConcept C159654299 @default.
- W22459633 hasConcept C203014093 @default.
- W22459633 hasConcept C2776409635 @default.
- W22459633 hasConcept C2776460866 @default.
- W22459633 hasConcept C2780832600 @default.
- W22459633 hasConcept C2910319489 @default.
- W22459633 hasConcept C45189115 @default.
- W22459633 hasConcept C49105822 @default.
- W22459633 hasConcept C54355233 @default.
- W22459633 hasConcept C55493867 @default.
- W22459633 hasConcept C71924100 @default.
- W22459633 hasConcept C86803240 @default.
- W22459633 hasConcept C89423630 @default.
- W22459633 hasConceptScore W22459633C104317684 @default.
- W22459633 hasConceptScore W22459633C159047783 @default.
- W22459633 hasConceptScore W22459633C159654299 @default.
- W22459633 hasConceptScore W22459633C203014093 @default.
- W22459633 hasConceptScore W22459633C2776409635 @default.
- W22459633 hasConceptScore W22459633C2776460866 @default.
- W22459633 hasConceptScore W22459633C2780832600 @default.
- W22459633 hasConceptScore W22459633C2910319489 @default.
- W22459633 hasConceptScore W22459633C45189115 @default.
- W22459633 hasConceptScore W22459633C49105822 @default.
- W22459633 hasConceptScore W22459633C54355233 @default.
- W22459633 hasConceptScore W22459633C55493867 @default.
- W22459633 hasConceptScore W22459633C71924100 @default.
- W22459633 hasConceptScore W22459633C86803240 @default.
- W22459633 hasConceptScore W22459633C89423630 @default.
- W22459633 hasIssue "4" @default.
- W22459633 hasLocation W224596331 @default.
- W22459633 hasOpenAccess W22459633 @default.
- W22459633 hasPrimaryLocation W224596331 @default.
- W22459633 hasRelatedWork W1945214198 @default.
- W22459633 hasRelatedWork W1975903633 @default.
- W22459633 hasRelatedWork W2026317280 @default.
- W22459633 hasRelatedWork W2046435329 @default.
- W22459633 hasRelatedWork W2059657252 @default.
- W22459633 hasRelatedWork W2064986005 @default.
- W22459633 hasRelatedWork W2066932010 @default.
- W22459633 hasRelatedWork W2144832848 @default.
- W22459633 hasRelatedWork W2416464813 @default.
- W22459633 hasRelatedWork W4233024077 @default.
- W22459633 hasVolume "50" @default.
- W22459633 isParatext "false" @default.
- W22459633 isRetracted "false" @default.
- W22459633 magId "22459633" @default.
- W22459633 workType "article" @default.