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- W2246179077 abstract "We evaluated and applied an existing faecal reproductive steroid extraction and radio-immunoassay (RIA) procedure to samples from captive short-beaked (Tachyglossus aculeatus) and long- beaked (Zaglossus sp.) echidnas. Steroids were extracted from faeces with diethyl ether, resuspended in 80% methanol and lipids removed with petroleum ether. The methanol fraction was purified and assayed for progestins or oestrogens, results corrected for procedural losses and converted to ng/ g dry weight of faeces. One T. aculeatus was injected with radiolabelled and natural progesterone and faecal extracts were subjected to high-performance liquid chromatography (HPLC) to allow partial identification of radiolabelled and RIA- reactive metabolites. The major RIA-reactive substance and the major labelled [14C] compound co-eluted with progesterone. An additional RIA-weak compound co-eluted with 20β-dihydroxyprogesterone, and three additional RIA-weak, radio-labelled compounds eluted but were not identified. Increases in faecal progestin of echidnas occurred at 17 3 (n = 5), 33 3 (n = 4) and 48 (n = 1) day intervals, supporting a cycle length of approximately 17 or 33 days. However, further study incorporating more animals, behavioural observations and more frequent sampling of faecal oestrogens is required to produce more definitive results." @default.
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- W2246179077 date "2004-02-20" @default.
- W2246179077 modified "2023-09-23" @default.
- W2246179077 title "Excretion Profiles of Some Reproductive Steroids in the Faeces of Captive Female Short-beaked Echidna (Tachyglossus aculeatus) and Long-beaked Echidna (Zaglossus sp.)" @default.
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