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- W2246244578 abstract "D-Tagatose production from D-galactose by an L-arabinose isomerase muntant from Geobacillus thermodenitrificans was performed with boric acid. The activity for maximum D-tagatose production was pH 9.0 and 60℃ with boric acid, and pH 8.5 and 65℃ without boric acid. The complexation of boric acid with D-tagatose was greater than with D-galactose in TLC. The conversion from D-galactose to D-tagatose was maximal at 40% (mM/mM) boric acid ratio when the ratio of boric acid to D-galactose was increased at 100 g/L, 200 g/L, and 300g/L D-galactose. The enzyme solution containing 40% (mM/mM) boric acid ratio and 300 g/L D-galactose produced 230 g/L tagatose at pH 8.5 and 60℃ for 20 h, corresponding to a conversion yield of 77% (w/w). The equilibrium shift toward D-tagatose at that ratio was due to the greatercomplexing capacity of boric acid to D-tagatose than D-galactose. The conversion yield obtained in this study is the highest reported value for D-tagatose production." @default.
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- W2246244578 date "2007-04-01" @default.
- W2246244578 modified "2023-09-23" @default.
- W2246244578 title "Equilibrium-shifted Production of D-Tagatose from D-Galactose by Selectivity of Boric Acid to the Sugars in an Enzymatic Process" @default.
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