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• W2246383445 abstract "Previous studies have suggested a beneficial effect ofdietary soy against inflammation and colon cancer. Much of this hasbeen attributed to soy isoflavones; however, soy proteins andpeptides have also shown bioactivity and antioxidant activity insome models. Enzymatic hydrolysis of proteins has also been shownto increase free radical scavenging by increasing solventaccessibility of active amino acids. We investigated theantioxidant capacity of an isoflavone-free soy protein concentrate(SPC) and an enzymatically-hydrolyzed SPC (SPH) using the oxygenradical absorbance capacity (ORAC) assay. Consistent with ourexpectations, SPH delayed the oxidation of fluoresceinsignificantly longer than SPC (p<0.01), indicating its superiorantioxidative capacity. The cytoprotective effects of SPC and SPHwere also compared in the Caco-2 human intestinal cell model toevaluate their ability to prevent H2O2-induced oxidation. Cellswere cotreated with 50 µM H2O2 and SPC or SPH (0-2 mg/ml) in PBSfor 60 min or were pretreated with SPC or SPH (0-2 mg/ml) for 30min and subsequently treated with 50 µM H2O2 for 60 min. Cellviability was assessed using the3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT)assay. Treatment with 0.5 mg/ml SPC increased cell viability from42.8-50.0% in H2O2-only treated cells to 76.1 ± 9.0% and 60.8 ±6.5% of the PBS control in the cotreatment and pretreatment trials,respectively. Higher concentrations of SPH were required to achievesimilar cytoprotective effects. At a concentration of 2 mg/ml SPH,cell viability was increased to 85.3 ± 10.9% and 62.1 ± 3.2% in thecotreatment and pretreatment trials, respectively. Photomicrographsof treated cells showed that SPC prevented H2O2-induced changes inmorphology and reduced intracellular reactive oxygen species (ROS).These effects were also observed for SPH treatment, but to a lesserextent. The effect of dietary supplementation with SPC on coloninflammation and carcinogenesis in male, CF-1 mice was investigatedin one 10-week and one 20-week study. In both studies, followingone week pretreatment with SPC or casein as the sole source ofprotein (19% kcal), mice were injected with the colon carcinogenazoxymethane (AOM, 10 mg/kg body wt). One week later, mice receiveddextran sodium sulfate (DSS, 1.5% w/v) as their sole drinking fluidfor one week to induce colon-specific inflammation. On days 3 and7, and weeks 7 and 20 (second study only) after DSS treatment, micewere euthanized and the colons were examined for markers ofinflammation and carcinogenesis. All samples were scored using theinflammation index, which measures inflammation area, severity,ulceration and hyperplasia and dysplasia. In both studies, trendsin these measures appeared to be highly dependent on sacrificetime-point. With the data from both studies combined, mean polypmultiplicity was significantly reduced in mice fed SPC at Week 7and for the study overall (p<0.001 and p<0.01, respectively).Hyperplasia and dysplasia, inflammation area and total…" @default.
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• W2246383445 date "2011-08-15" @default.
• W2246383445 modified "2023-09-27" @default.
• W2246383445 title "The Effect of Soy Protein on Colon Carcinogenesis in vitro and in vivo" @default.
• W2246383445 hasPublicationYear "2011" @default.
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