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• W2247003600 abstract "Summary Interspecific crosses between Phaseolus vulgaris L. and P. coccineus L. almost always lead to embryo abortions at the globular stage. It is possible to rescue them using the pod culture technique, but this produces a very low regeneration rate (2.8%) due to poor root formation. In order to improve the regeneration of P. coccineus x P. vulgaris hybrid embryos, various tests were conducted with the aim of developing a micropropagation technique using microcuttings from cotyledonary nodes. The influence of the mineral composition of the culture medium and the addition of activated charcoal were evaluated as a means of promoting rooting. These tests served to evaluate the influence of the mineral composition of the culture medium and the addition of activated charcoal to the latter, in terms of encouraging rooting. The reaction of various genotypes to this technique was also evaluated. The use of a modified MS medium made it possible to produce plantlets with roots from 93% of cultivated explants of the NI16 variety of P. coccineus, while no regeneration took place when the plantlets were cultivated using a modified B5 Gamborg medium. It was possible to acclimate all plantlets produced on the modified MS medium. Micropropagation using microcuttings is more effective with P. coccineus (73.33% regeneration) than P. vulgaris (± 50%). The activated charcoal found in the modified MS medium made it possible to double the rate of regeneration from microcuttings in P. vulgaris. Pod culture combined with micropropagation using microcuttings from cotyledonary nodes achieved better results than regeneration methods using only pod culture. This new protocol opens up a new and promising method of rescuing embryos that would otherwise abort at the globular stage following interspecific hybridization using P. coccineus as the female parent." @default.
• W2247003600 created "2016-06-24" @default.
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• W2247003600 date "2011-01-01" @default.
• W2247003600 modified "2023-09-28" @default.
• W2247003600 title "refinement of an in vitro Culture Technique for the Rescue of Globular Embryos Using Microcutting for P. vulgaris L. and P. coccineus L." @default.
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