Matches in SemOpenAlex for { <https://semopenalex.org/work/W2247144981> ?p ?o ?g. }
- W2247144981 abstract "Cell-free environments are becoming viable alternatives for implementing biological networks in synthetic biology. The reconstituted cell-free expression system (PURE) allows characterization of genetic networks under defined conditions but its applicability to native bacterial promoters and endogenous genetic networks is limited due to the poor transcription rate of Escherichia coli RNA polymerase in this minimal system. We found that addition of transcription elongation factors GreA and GreB to the PURE system increased transcription rates of E. coli RNA polymerase from sigma factor 70 promoters up to 6-fold and enhanced the performance of a genetic network. Furthermore, we reconstituted activation of natural E. coli promoters controlling flagella biosynthesis by the transcriptional activator FlhDC and sigma factor 28. Addition of GreA/GreB to the PURE system allows efficient expression from natural and synthetic E. coli promoters and characterization of their regulation in minimal and defined reaction conditions making the PURE system more broadly applicable to study genetic networks and bottom-up synthetic biology." @default.
- W2247144981 created "2016-06-24" @default.
- W2247144981 creator A5006811260 @default.
- W2247144981 creator A5031110778 @default.
- W2247144981 creator A5032517726 @default.
- W2247144981 creator A5039101195 @default.
- W2247144981 creator A5067492574 @default.
- W2247144981 creator A5084128215 @default.
- W2247144981 date "2015-08-13" @default.
- W2247144981 modified "2023-09-26" @default.
- W2247144981 title "GreA and GreB enhanceEscherichia coliRNA polymerase transcription rate in a reconstituted transcription-translation system" @default.
- W2247144981 cites W1569060300 @default.
- W2247144981 cites W1587481053 @default.
- W2247144981 cites W1825825356 @default.
- W2247144981 cites W1973256834 @default.
- W2247144981 cites W1973873896 @default.
- W2247144981 cites W1975379503 @default.
- W2247144981 cites W1978151264 @default.
- W2247144981 cites W1982228455 @default.
- W2247144981 cites W1983913689 @default.
- W2247144981 cites W1991605266 @default.
- W2247144981 cites W1998691299 @default.
- W2247144981 cites W2009616516 @default.
- W2247144981 cites W2016065853 @default.
- W2247144981 cites W2023368756 @default.
- W2247144981 cites W2023955197 @default.
- W2247144981 cites W2025166348 @default.
- W2247144981 cites W2026612033 @default.
- W2247144981 cites W2038540528 @default.
- W2247144981 cites W2060528969 @default.
- W2247144981 cites W2067167529 @default.
- W2247144981 cites W2071463351 @default.
- W2247144981 cites W2075284797 @default.
- W2247144981 cites W2080233018 @default.
- W2247144981 cites W2087993212 @default.
- W2247144981 cites W2090392380 @default.
- W2247144981 cites W2097779143 @default.
- W2247144981 cites W2098037868 @default.
- W2247144981 cites W2101703936 @default.
- W2247144981 cites W2103053013 @default.
- W2247144981 cites W2106487742 @default.
- W2247144981 cites W2121806138 @default.
- W2247144981 cites W2121998297 @default.
- W2247144981 cites W2139161084 @default.
- W2247144981 cites W2147775831 @default.
- W2247144981 cites W2152066012 @default.
- W2247144981 cites W2153067536 @default.
- W2247144981 cites W2161307281 @default.
- W2247144981 cites W2161782181 @default.
- W2247144981 cites W2170806980 @default.
- W2247144981 cites W2327010950 @default.
- W2247144981 cites W2400779101 @default.
- W2247144981 cites W2467580774 @default.
- W2247144981 cites W2950851734 @default.
- W2247144981 cites W2124013800 @default.
- W2247144981 doi "https://doi.org/10.1101/024604" @default.
- W2247144981 hasPublicationYear "2015" @default.
- W2247144981 type Work @default.
- W2247144981 sameAs 2247144981 @default.
- W2247144981 citedByCount "0" @default.
- W2247144981 crossrefType "posted-content" @default.
- W2247144981 hasAuthorship W2247144981A5006811260 @default.
- W2247144981 hasAuthorship W2247144981A5031110778 @default.
- W2247144981 hasAuthorship W2247144981A5032517726 @default.
- W2247144981 hasAuthorship W2247144981A5039101195 @default.
- W2247144981 hasAuthorship W2247144981A5067492574 @default.
- W2247144981 hasAuthorship W2247144981A5084128215 @default.
- W2247144981 hasBestOaLocation W22471449811 @default.
- W2247144981 hasConcept C101762097 @default.
- W2247144981 hasConcept C104317684 @default.
- W2247144981 hasConcept C138885662 @default.
- W2247144981 hasConcept C150194340 @default.
- W2247144981 hasConcept C179926584 @default.
- W2247144981 hasConcept C191908910 @default.
- W2247144981 hasConcept C2776449523 @default.
- W2247144981 hasConcept C41895202 @default.
- W2247144981 hasConcept C47319516 @default.
- W2247144981 hasConcept C54355233 @default.
- W2247144981 hasConcept C547475151 @default.
- W2247144981 hasConcept C70721500 @default.
- W2247144981 hasConcept C86339819 @default.
- W2247144981 hasConcept C86803240 @default.
- W2247144981 hasConcept C88045685 @default.
- W2247144981 hasConceptScore W2247144981C101762097 @default.
- W2247144981 hasConceptScore W2247144981C104317684 @default.
- W2247144981 hasConceptScore W2247144981C138885662 @default.
- W2247144981 hasConceptScore W2247144981C150194340 @default.
- W2247144981 hasConceptScore W2247144981C179926584 @default.
- W2247144981 hasConceptScore W2247144981C191908910 @default.
- W2247144981 hasConceptScore W2247144981C2776449523 @default.
- W2247144981 hasConceptScore W2247144981C41895202 @default.
- W2247144981 hasConceptScore W2247144981C47319516 @default.
- W2247144981 hasConceptScore W2247144981C54355233 @default.
- W2247144981 hasConceptScore W2247144981C547475151 @default.
- W2247144981 hasConceptScore W2247144981C70721500 @default.
- W2247144981 hasConceptScore W2247144981C86339819 @default.
- W2247144981 hasConceptScore W2247144981C86803240 @default.
- W2247144981 hasConceptScore W2247144981C88045685 @default.
- W2247144981 hasLocation W22471449811 @default.
- W2247144981 hasOpenAccess W2247144981 @default.