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- W2247211989 abstract "Objective To compare sensitivity and specificity of gold-immunochromatography assay(GICA) and double monoclonal antibody sandwich enzyme-linked immunosorbent assay(DMcAbS-ELISA) for detecting F1 antigen of Yersinia pestis.Methods Viscera organ(liver and spleen) specimens of 308 mice with virulent Yersinia pestis infection and 327 control samples of rats were detected with GICA dipstick and DMcAbS-ELISA.Meanwhile,micro-method of reverse indirect hemagglutination assay(RIHA) was carried out for parallel testing.Results Plague bacteriological tests of 327 control rats,F1 antigen detection with GICA,DMcAbS-ELISA and RIHA were negative.No cross-reaction with relatives of Yersinia pseudotuberculosis at 108 cfu/mL level was found in GICA,DMcAbS-ELISA and RIHA.Out of the 308 infected mice,284 were positive of plague bacterial culture,24 samples without isolation of Yersinia pestis.There were 287 of positive by GICA,280 of positive by DMcAbS-ELISA,276 of positive by RIHA.Positive detection rate of GICA was the highest of 93.19%,with statistically significant differences when compared with DMcAbS-ELISA and RIHA(χ2=5.14,9.09;P=0.016,0.001).Coincidence was 97.73% between GICA and DMcAbS-ELISA(Kappa=0.845),and 96.43% and 98.70% between GICA,DMcAbS-ELISA and RIHA,respectively(Kappa = 0.774,0.926).In 284 samples with positive bacterial culture,detection rate of F1 antigen was 98.59% by GICA,higher than that by DMcAbS-ELISA and RIHA,with statistically significant difference(χ2=4.17,5.14;P=0.031,0.016).Out of 24 specimens with negative bacterial culture for F1 antigen,there were 7 of positive detected by GICA with the positive rate of 29.17%,6 of positive by DMcAbS-ELISA with the positive rate of 25%,3 of positive by RIHA with the positive rate of 12.5%.The positive rate of GICA was higher than that of DMcAbS-ELISA and RIHA,but no statistically significant difference was found(χ2 = 2.25,0;P=0.125,1.000).Conclusions GICA is sensitive and specific,fast and simple in detection for F1 antigen of plague,better than DMcAbS-ELISA and RIHA.It's valuable detection technique for early and rapid diagnosis of plague." @default.
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- W2247211989 date "2010-01-01" @default.
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- W2247211989 title "Comparison of Detecting F1 Antigen of Yersinia pestis Between Gold-immunochromatography Assay and Double Monoclonal Antibody Sandwich Enzyme-linked Immunosorbent Assay" @default.
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