Matches in SemOpenAlex for { <https://semopenalex.org/work/W2247299364> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W2247299364 endingPage "551" @default.
- W2247299364 startingPage "550" @default.
- W2247299364 abstract "The simplicity and diversity of viruses makes characteristic viral molecules difficult for the innate immune system to define. Activation by viral double stranded (ds) RNA has been known for many years, and this occurs through both the endosomal receptor TLR3 and cytoplasmic receptors. Recently other nucleic acids have emerged as important indicators of viral infection: CpG motif-containing DNA and viral single stranded (ss) RNAs are recognised in the endosomal compartment by TLR9 and TLR7 respectively. We propose a novel mechanism exists for recognition of viral dsDNA. Macrophages respond to transfected ds but not ssDNA with MHC I upregulation, secretion of IL-6 and TNF-a, and apoptotic death. In primary macrophages, death following electroporation is profound, and is dsDNA dependent. Cell death is independent of TLR9. The effect is sequence-independent, but dependent on the length of DNA, with maximal death seen above 1 kb. Activation of caspase 3 within 10 minutes after electroporation with dsDNA shows that there is a specific rapid response to transfected DNA. Relevance of this phenomenon to anti-viral defence is suggested by published work showing that retroviral superinfections resulting in accumulation of unintegrated cytoplasmic DNA cause apoptosis. Although DNA damage pathways could be involved in the response, we have found that p53, DNA-PK and ATM functions are not required for cell death. We are characterising sequence non-specific DNA binding proteins as potential receptors for foreign dsDNA. Apart from possible relevance to detection of pathogen DNA, elucidation of this pathway is relevant to TLR9-independent cellular activation in DNA vaccination and improving gene therapy." @default.
- W2247299364 created "2016-06-24" @default.
- W2247299364 creator A5036256886 @default.
- W2247299364 creator A5043947319 @default.
- W2247299364 creator A5046551226 @default.
- W2247299364 creator A5056733821 @default.
- W2247299364 creator A5065478570 @default.
- W2247299364 creator A5085580367 @default.
- W2247299364 date "2005-01-01" @default.
- W2247299364 modified "2023-09-28" @default.
- W2247299364 title "A detection system for viral dsDNA" @default.
- W2247299364 hasPublicationYear "2005" @default.
- W2247299364 type Work @default.
- W2247299364 sameAs 2247299364 @default.
- W2247299364 citedByCount "0" @default.
- W2247299364 crossrefType "journal-article" @default.
- W2247299364 hasAuthorship W2247299364A5036256886 @default.
- W2247299364 hasAuthorship W2247299364A5043947319 @default.
- W2247299364 hasAuthorship W2247299364A5046551226 @default.
- W2247299364 hasAuthorship W2247299364A5056733821 @default.
- W2247299364 hasAuthorship W2247299364A5065478570 @default.
- W2247299364 hasAuthorship W2247299364A5085580367 @default.
- W2247299364 hasConcept C102747710 @default.
- W2247299364 hasConcept C104317684 @default.
- W2247299364 hasConcept C111289621 @default.
- W2247299364 hasConcept C136449434 @default.
- W2247299364 hasConcept C153911025 @default.
- W2247299364 hasConcept C170493617 @default.
- W2247299364 hasConcept C2776709828 @default.
- W2247299364 hasConcept C2779836521 @default.
- W2247299364 hasConcept C54009773 @default.
- W2247299364 hasConcept C54355233 @default.
- W2247299364 hasConcept C552990157 @default.
- W2247299364 hasConcept C79879829 @default.
- W2247299364 hasConcept C86803240 @default.
- W2247299364 hasConcept C95444343 @default.
- W2247299364 hasConceptScore W2247299364C102747710 @default.
- W2247299364 hasConceptScore W2247299364C104317684 @default.
- W2247299364 hasConceptScore W2247299364C111289621 @default.
- W2247299364 hasConceptScore W2247299364C136449434 @default.
- W2247299364 hasConceptScore W2247299364C153911025 @default.
- W2247299364 hasConceptScore W2247299364C170493617 @default.
- W2247299364 hasConceptScore W2247299364C2776709828 @default.
- W2247299364 hasConceptScore W2247299364C2779836521 @default.
- W2247299364 hasConceptScore W2247299364C54009773 @default.
- W2247299364 hasConceptScore W2247299364C54355233 @default.
- W2247299364 hasConceptScore W2247299364C552990157 @default.
- W2247299364 hasConceptScore W2247299364C79879829 @default.
- W2247299364 hasConceptScore W2247299364C86803240 @default.
- W2247299364 hasConceptScore W2247299364C95444343 @default.
- W2247299364 hasIssue "5" @default.
- W2247299364 hasLocation W22472993641 @default.
- W2247299364 hasOpenAccess W2247299364 @default.
- W2247299364 hasPrimaryLocation W22472993641 @default.
- W2247299364 hasRelatedWork W1905052514 @default.
- W2247299364 hasRelatedWork W1934619019 @default.
- W2247299364 hasRelatedWork W1963440056 @default.
- W2247299364 hasRelatedWork W2006025022 @default.
- W2247299364 hasRelatedWork W2035858672 @default.
- W2247299364 hasRelatedWork W2040318657 @default.
- W2247299364 hasRelatedWork W2051186727 @default.
- W2247299364 hasRelatedWork W2068617051 @default.
- W2247299364 hasRelatedWork W2072171296 @default.
- W2247299364 hasRelatedWork W2094793968 @default.
- W2247299364 hasRelatedWork W2109639180 @default.
- W2247299364 hasRelatedWork W2124969332 @default.
- W2247299364 hasRelatedWork W2152998216 @default.
- W2247299364 hasRelatedWork W2240523383 @default.
- W2247299364 hasRelatedWork W2254125592 @default.
- W2247299364 hasRelatedWork W2564721597 @default.
- W2247299364 hasRelatedWork W2938509953 @default.
- W2247299364 hasRelatedWork W2969613484 @default.
- W2247299364 hasRelatedWork W86836483 @default.
- W2247299364 hasRelatedWork W92757221 @default.
- W2247299364 hasVolume "66" @default.
- W2247299364 isParatext "false" @default.
- W2247299364 isRetracted "false" @default.
- W2247299364 magId "2247299364" @default.
- W2247299364 workType "article" @default.