Matches in SemOpenAlex for { <https://semopenalex.org/work/W2247348355> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W2247348355 endingPage "110" @default.
- W2247348355 startingPage "100" @default.
- W2247348355 abstract "Background: Sustainable and commercial production of taxol as an anti cancer drug is a critical point to its clinical application. Nowadays, hazel because of rapid growth and wide range distribution is considered as an alternative source of Taxol. Objective: To increase taxol production the cDNA encoding 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) from Iranian hazel (GeneBank accession number KF306244, showed by CiHMGR) was isolated and over-expressed in pCAMBIA1304 binary vector. The effect of transient over-expression of HMGR in callus and leaf were evaluated on Taxol production. Methods: The calli was established through the culture of immature cotyledon on Murashige and Skoog basal medium supplemented with 2, 4-D and BA. The first strand cDNA of CiHMGR was synthesized by specific primers. Enzymatic assay of recombinant CiHMGR in E. coli were done by western blott and His-tag affinity techniques. Also production of taxol in transformed callus and leaf were evaluated by HPLC analysis. Results: An Open Reading Frame (ORF) with 1698 bp length and a deduced polypeptide with 566 amino acid residues were amplified. The highest and lowest amount of taxol was 0.016 mg/g.DW and 0.004 mg/gDW in transformed calli and untransformed leaves respectively. Conclusion: Generally the over-expression of HMGR increase the total isoprenoids yield, therefore to have high production of target secondary metabolites (taxol) we need both of network of transformed genes and elicited cell culture." @default.
- W2247348355 created "2016-06-24" @default.
- W2247348355 creator A5000831408 @default.
- W2247348355 creator A5015079596 @default.
- W2247348355 creator A5051044091 @default.
- W2247348355 creator A5082171674 @default.
- W2247348355 date "2013-09-10" @default.
- W2247348355 modified "2023-09-23" @default.
- W2247348355 title "OVER - EXPRESSION EFFECT OF GENE ENCODING 3-HYDROXY-3-METHYLGLUTARYL-COA REDUCTASE ON PRODUCTION OF TAXOL IN IRANIAN HAZEL (CORYLUS AVELLANA L.)" @default.
- W2247348355 cites W1017840660 @default.
- W2247348355 cites W1493515436 @default.
- W2247348355 cites W1607051768 @default.
- W2247348355 cites W1916975800 @default.
- W2247348355 cites W1964973869 @default.
- W2247348355 cites W1974842306 @default.
- W2247348355 cites W1981152389 @default.
- W2247348355 cites W1994977506 @default.
- W2247348355 cites W2013966401 @default.
- W2247348355 cites W2015778839 @default.
- W2247348355 cites W2024556021 @default.
- W2247348355 cites W2036886024 @default.
- W2247348355 cites W2061110431 @default.
- W2247348355 cites W2107637490 @default.
- W2247348355 cites W2112556227 @default.
- W2247348355 cites W2118161743 @default.
- W2247348355 cites W2124919759 @default.
- W2247348355 cites W2125671793 @default.
- W2247348355 cites W2126667730 @default.
- W2247348355 cites W2131253747 @default.
- W2247348355 cites W2142793279 @default.
- W2247348355 cites W2156713073 @default.
- W2247348355 cites W2159482845 @default.
- W2247348355 cites W2488652735 @default.
- W2247348355 cites W2952827588 @default.
- W2247348355 cites W3110148814 @default.
- W2247348355 cites W576642463 @default.
- W2247348355 hasPublicationYear "2013" @default.
- W2247348355 type Work @default.
- W2247348355 sameAs 2247348355 @default.
- W2247348355 citedByCount "0" @default.
- W2247348355 crossrefType "journal-article" @default.
- W2247348355 hasAuthorship W2247348355A5000831408 @default.
- W2247348355 hasAuthorship W2247348355A5015079596 @default.
- W2247348355 hasAuthorship W2247348355A5051044091 @default.
- W2247348355 hasAuthorship W2247348355A5082171674 @default.
- W2247348355 hasConcept C104317684 @default.
- W2247348355 hasConcept C134651460 @default.
- W2247348355 hasConcept C167625842 @default.
- W2247348355 hasConcept C181199279 @default.
- W2247348355 hasConcept C185476388 @default.
- W2247348355 hasConcept C187882448 @default.
- W2247348355 hasConcept C189482833 @default.
- W2247348355 hasConcept C47289529 @default.
- W2247348355 hasConcept C55493867 @default.
- W2247348355 hasConcept C59822182 @default.
- W2247348355 hasConcept C86803240 @default.
- W2247348355 hasConcept C9549007 @default.
- W2247348355 hasConceptScore W2247348355C104317684 @default.
- W2247348355 hasConceptScore W2247348355C134651460 @default.
- W2247348355 hasConceptScore W2247348355C167625842 @default.
- W2247348355 hasConceptScore W2247348355C181199279 @default.
- W2247348355 hasConceptScore W2247348355C185476388 @default.
- W2247348355 hasConceptScore W2247348355C187882448 @default.
- W2247348355 hasConceptScore W2247348355C189482833 @default.
- W2247348355 hasConceptScore W2247348355C47289529 @default.
- W2247348355 hasConceptScore W2247348355C55493867 @default.
- W2247348355 hasConceptScore W2247348355C59822182 @default.
- W2247348355 hasConceptScore W2247348355C86803240 @default.
- W2247348355 hasConceptScore W2247348355C9549007 @default.
- W2247348355 hasIssue "47" @default.
- W2247348355 hasOpenAccess W2247348355 @default.
- W2247348355 hasRelatedWork W1981696774 @default.
- W2247348355 hasRelatedWork W2009796288 @default.
- W2247348355 hasRelatedWork W2020517269 @default.
- W2247348355 hasRelatedWork W2045990270 @default.
- W2247348355 hasRelatedWork W2046861930 @default.
- W2247348355 hasRelatedWork W2067421976 @default.
- W2247348355 hasRelatedWork W2070252709 @default.
- W2247348355 hasRelatedWork W2120878876 @default.
- W2247348355 hasRelatedWork W2128040434 @default.
- W2247348355 hasRelatedWork W2152038789 @default.
- W2247348355 hasRelatedWork W2287050394 @default.
- W2247348355 hasRelatedWork W2511983109 @default.
- W2247348355 hasRelatedWork W2761397075 @default.
- W2247348355 hasRelatedWork W2905042531 @default.
- W2247348355 hasRelatedWork W2921686266 @default.
- W2247348355 hasRelatedWork W2951081529 @default.
- W2247348355 hasRelatedWork W2980981300 @default.
- W2247348355 hasRelatedWork W2998921392 @default.
- W2247348355 hasRelatedWork W3009368777 @default.
- W2247348355 hasRelatedWork W829549238 @default.
- W2247348355 hasVolume "12" @default.
- W2247348355 isParatext "false" @default.
- W2247348355 isRetracted "false" @default.
- W2247348355 magId "2247348355" @default.
- W2247348355 workType "article" @default.