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- W2247354372 abstract "Abstract. Bioprocesses can be utilized for the sustainable production of biofuels and other value-added co-products. However, most bioprocesses targeting production of low value, high-volume products cannot yet compete with conventional technologies and require further development. On-line monitoring can help expedite bioprocess development by allowing dynamic study of microbial metabolism, optimal process control, and product quality assurance. Titrimetric off-gas analysis (TOGA) is an on-line sensor system that combines a membrane inlet mass spectrometer (MIMS) with on-line titration techniques to allow dynamic study of biological processes, including fermentation. In this study, TOGA was applied to fermentation of 2 g L -1 cellobiose with Clostridium thermocellum. Ethanol, CO 2 , H 2 , and total hydrogen ion production (HP) were quantified in real time. Concurrent off-line analysis was performed for verification using high-performance liquid chromatography and gas chromatography. Errors in the on-line data with respect to off-line analyses were found to be 4.8% ±3.7%, 7.1% ±3.5%, and 10.7% ±9.0% for H 2 , CO 2 , and ethanol, respectively. The discrepancies between the on-line and off-line analyses were not statistically significant with 95% confidence intervals. The titration system effectively maintained the process pH at 7.2 ±0.02 and also measured the hydrogen ion production (HP), which could be 88.4% accounted for in the production of CO 2 and organic acids, as determined by off-line analyses. The instrument was shown to be a viable on-line sensor that can monitor multiple physiologically relevant variables and contribute insight to the development and control of fermentation with C. thermocellum. The methodology can easily be extended to other fermentation processes." @default.
- W2247354372 created "2016-06-24" @default.
- W2247354372 date "2014-05-30" @default.
- W2247354372 modified "2023-10-17" @default.
- W2247354372 title "Real-Time Monitoring of Microbial Fermentation End-Products in Biofuel Production with Titrimetric Off-Gas Analysis (TOGA)" @default.
- W2247354372 doi "https://doi.org/10.13031/bet6.10496" @default.
- W2247354372 hasPublicationYear "2014" @default.
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