Matches in SemOpenAlex for { <https://semopenalex.org/work/W2247395277> ?p ?o ?g. }
Showing items 1 to 86 of
86
with 100 items per page.
- W2247395277 abstract "Epigenetic gene inactivation of tumour suppressor genes underlies several human cancers. The potential to develop targeted therapies is being driven by the recent development of high-throughput genome-wide approaches including methylation-based microarrays. Here we report a large-scale project aiming to characterize the lung cancer methylome.Methods: Methylation profiling by Illumina Infinium Methylation27 arrays was performed on 226 primary lung tumours and 81 normal lung samples from the TPCH lung tumour bank. Data was feature extracted using proprietary software (Genome Studio, Illumina, Hayward, CA). We used two separate approaches in gene identification: 1) we utilised stringent criteria employed by the The Cancer Genome Atlas (TCGA) project on Glioblastoma performing separate analyses for training and test sets and 2) we used in-house (TPCH) criteria (vis. (1) false discovery rate (FDR) P<1x10E-7, (2) P-value < 1x10E-7, (3) frequency of methylation across samples and (4) magnitude of difference between normal/tumour pairs (at least 2-fold)).Results: Unsupervised cluster analyses demonstrated distinct clusters for normal and tumour lung and in primary tumours identified six distinct tumour clades which could not be explained purely by histology. Differential methylation analysis identified 171 CpG loci highly methylated in tumours exhibiting substantially higher methylation levels compared to common lung cancer methylation markers including P16, MGMT and DAPK1. Next, we demonstrate samples frequently methylated across multiple genes demonstrate a survival advantage. Finally, we report methylation signatures capable of distinguishing adenocarcinoma and squamous cell carcinoma histologies.Discussion: DNA methylation profiles differ between normal and tumour lung and are predictive of adenocarcinoma and squamous cell carcinoma histology. Further validation studies are required to confirm involvement of candidate genes in lung carcinogenesis." @default.
- W2247395277 created "2016-06-24" @default.
- W2247395277 creator A5004321369 @default.
- W2247395277 creator A5021524687 @default.
- W2247395277 creator A5036158579 @default.
- W2247395277 creator A5041417395 @default.
- W2247395277 creator A5054380183 @default.
- W2247395277 creator A5066853533 @default.
- W2247395277 creator A5069706679 @default.
- W2247395277 creator A5079283705 @default.
- W2247395277 creator A5080037744 @default.
- W2247395277 date "2011-01-01" @default.
- W2247395277 modified "2023-09-27" @default.
- W2247395277 title "Mapping the lung cancer methylome" @default.
- W2247395277 hasPublicationYear "2011" @default.
- W2247395277 type Work @default.
- W2247395277 sameAs 2247395277 @default.
- W2247395277 citedByCount "0" @default.
- W2247395277 crossrefType "journal-article" @default.
- W2247395277 hasAuthorship W2247395277A5004321369 @default.
- W2247395277 hasAuthorship W2247395277A5021524687 @default.
- W2247395277 hasAuthorship W2247395277A5036158579 @default.
- W2247395277 hasAuthorship W2247395277A5041417395 @default.
- W2247395277 hasAuthorship W2247395277A5054380183 @default.
- W2247395277 hasAuthorship W2247395277A5066853533 @default.
- W2247395277 hasAuthorship W2247395277A5069706679 @default.
- W2247395277 hasAuthorship W2247395277A5079283705 @default.
- W2247395277 hasAuthorship W2247395277A5080037744 @default.
- W2247395277 hasConcept C104317684 @default.
- W2247395277 hasConcept C121608353 @default.
- W2247395277 hasConcept C140173407 @default.
- W2247395277 hasConcept C143998085 @default.
- W2247395277 hasConcept C150194340 @default.
- W2247395277 hasConcept C190727270 @default.
- W2247395277 hasConcept C2776256026 @default.
- W2247395277 hasConcept C2781182431 @default.
- W2247395277 hasConcept C33288867 @default.
- W2247395277 hasConcept C41091548 @default.
- W2247395277 hasConcept C502942594 @default.
- W2247395277 hasConcept C54355233 @default.
- W2247395277 hasConcept C70721500 @default.
- W2247395277 hasConcept C71924100 @default.
- W2247395277 hasConcept C86803240 @default.
- W2247395277 hasConceptScore W2247395277C104317684 @default.
- W2247395277 hasConceptScore W2247395277C121608353 @default.
- W2247395277 hasConceptScore W2247395277C140173407 @default.
- W2247395277 hasConceptScore W2247395277C143998085 @default.
- W2247395277 hasConceptScore W2247395277C150194340 @default.
- W2247395277 hasConceptScore W2247395277C190727270 @default.
- W2247395277 hasConceptScore W2247395277C2776256026 @default.
- W2247395277 hasConceptScore W2247395277C2781182431 @default.
- W2247395277 hasConceptScore W2247395277C33288867 @default.
- W2247395277 hasConceptScore W2247395277C41091548 @default.
- W2247395277 hasConceptScore W2247395277C502942594 @default.
- W2247395277 hasConceptScore W2247395277C54355233 @default.
- W2247395277 hasConceptScore W2247395277C70721500 @default.
- W2247395277 hasConceptScore W2247395277C71924100 @default.
- W2247395277 hasConceptScore W2247395277C86803240 @default.
- W2247395277 hasLocation W22473952771 @default.
- W2247395277 hasOpenAccess W2247395277 @default.
- W2247395277 hasPrimaryLocation W22473952771 @default.
- W2247395277 hasRelatedWork W1490563308 @default.
- W2247395277 hasRelatedWork W1524744095 @default.
- W2247395277 hasRelatedWork W1673191603 @default.
- W2247395277 hasRelatedWork W1924537943 @default.
- W2247395277 hasRelatedWork W1972060232 @default.
- W2247395277 hasRelatedWork W1993492315 @default.
- W2247395277 hasRelatedWork W2024438850 @default.
- W2247395277 hasRelatedWork W2072202929 @default.
- W2247395277 hasRelatedWork W2085900506 @default.
- W2247395277 hasRelatedWork W2106057638 @default.
- W2247395277 hasRelatedWork W2133646854 @default.
- W2247395277 hasRelatedWork W2327501393 @default.
- W2247395277 hasRelatedWork W2329531764 @default.
- W2247395277 hasRelatedWork W2619521340 @default.
- W2247395277 hasRelatedWork W2800639479 @default.
- W2247395277 hasRelatedWork W2952191603 @default.
- W2247395277 hasRelatedWork W2957423565 @default.
- W2247395277 hasRelatedWork W3046000184 @default.
- W2247395277 hasRelatedWork W3133862682 @default.
- W2247395277 hasRelatedWork W2182462813 @default.
- W2247395277 hasVolume "6" @default.
- W2247395277 isParatext "false" @default.
- W2247395277 isRetracted "false" @default.
- W2247395277 magId "2247395277" @default.
- W2247395277 workType "article" @default.