Matches in SemOpenAlex for { <https://semopenalex.org/work/W2247398408> ?p ?o ?g. }
- W2247398408 endingPage "19196" @default.
- W2247398408 startingPage "19184" @default.
- W2247398408 abstract "VhChiP is a sugar-specific porin present in the outer membrane of the marine bacterium Vibrio harveyi. VhChiP is responsible for the uptake of chitin oligosaccharides, with particular selectivity for chitohexaose. In this study, we employed electrophysiological and biochemical approaches to demonstrate that Trp136, located at the mouth of the VhChiP pore, plays an essential role in controlling the channel's ion conductivity, chitin affinity, and permeability. Kinetic analysis of sugar translocation obtained from single channel recordings indicated that the Trp136 mutations W136A, W136D, W136R, and W136F considerably reduce the binding affinity of the protein channel for its best substrate, chitohexaose. Liposome swelling assays confirmed that the Trp136 mutations decreased the rate of bulk chitohexaose permeation through the VhChiP channel. Notably, all of the mutants show increases in the off-rate for chitohexaose of up to 20-fold compared with that of the native channel. Furthermore, the cation/anion permeability ratio Pc/Pa is decreased in the W136R mutant and increased in the W136D mutant. This demonstrates that the negatively charged surface at the interior of the protein lumen preferentially attracts cationic species, leading to the cation selectivity of this trimeric channel." @default.
- W2247398408 created "2016-06-24" @default.
- W2247398408 creator A5010991829 @default.
- W2247398408 creator A5067591268 @default.
- W2247398408 creator A5069862319 @default.
- W2247398408 creator A5080089165 @default.
- W2247398408 creator A5087056656 @default.
- W2247398408 date "2015-07-01" @default.
- W2247398408 modified "2023-10-13" @default.
- W2247398408 title "Chitoporin from the Marine Bacterium Vibrio harveyi" @default.
- W2247398408 cites W1648158953 @default.
- W2247398408 cites W1885615951 @default.
- W2247398408 cites W1964080252 @default.
- W2247398408 cites W1965824205 @default.
- W2247398408 cites W1969479499 @default.
- W2247398408 cites W1971554935 @default.
- W2247398408 cites W1973468358 @default.
- W2247398408 cites W1983009138 @default.
- W2247398408 cites W1984717522 @default.
- W2247398408 cites W1986651499 @default.
- W2247398408 cites W1987622073 @default.
- W2247398408 cites W1995859158 @default.
- W2247398408 cites W1996332682 @default.
- W2247398408 cites W1998109302 @default.
- W2247398408 cites W2000870210 @default.
- W2247398408 cites W2007599036 @default.
- W2247398408 cites W2019447533 @default.
- W2247398408 cites W2025499119 @default.
- W2247398408 cites W2026582002 @default.
- W2247398408 cites W2029648392 @default.
- W2247398408 cites W2039505373 @default.
- W2247398408 cites W2047412060 @default.
- W2247398408 cites W2047668003 @default.
- W2247398408 cites W2048791854 @default.
- W2247398408 cites W2052593063 @default.
- W2247398408 cites W2059711876 @default.
- W2247398408 cites W2070695325 @default.
- W2247398408 cites W2081636992 @default.
- W2247398408 cites W2084283959 @default.
- W2247398408 cites W2087116956 @default.
- W2247398408 cites W2093293698 @default.
- W2247398408 cites W2096419043 @default.
- W2247398408 cites W2100507403 @default.
- W2247398408 cites W2105853147 @default.
- W2247398408 cites W2117819577 @default.
- W2247398408 cites W2121794369 @default.
- W2247398408 cites W2121924211 @default.
- W2247398408 cites W2127881473 @default.
- W2247398408 cites W2156365482 @default.
- W2247398408 cites W2168950191 @default.
- W2247398408 cites W2312193024 @default.
- W2247398408 cites W2395905059 @default.
- W2247398408 doi "https://doi.org/10.1074/jbc.m115.660530" @default.
- W2247398408 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4521040" @default.
- W2247398408 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26082491" @default.
- W2247398408 hasPublicationYear "2015" @default.
- W2247398408 type Work @default.
- W2247398408 sameAs 2247398408 @default.
- W2247398408 citedByCount "16" @default.
- W2247398408 countsByYear W22473984082016 @default.
- W2247398408 countsByYear W22473984082018 @default.
- W2247398408 countsByYear W22473984082019 @default.
- W2247398408 countsByYear W22473984082020 @default.
- W2247398408 countsByYear W22473984082021 @default.
- W2247398408 countsByYear W22473984082022 @default.
- W2247398408 countsByYear W22473984082023 @default.
- W2247398408 crossrefType "journal-article" @default.
- W2247398408 hasAuthorship W2247398408A5010991829 @default.
- W2247398408 hasAuthorship W2247398408A5067591268 @default.
- W2247398408 hasAuthorship W2247398408A5069862319 @default.
- W2247398408 hasAuthorship W2247398408A5080089165 @default.
- W2247398408 hasAuthorship W2247398408A5087056656 @default.
- W2247398408 hasBestOaLocation W22473984081 @default.
- W2247398408 hasConcept C153568844 @default.
- W2247398408 hasConcept C185592680 @default.
- W2247398408 hasConcept C2776154503 @default.
- W2247398408 hasConcept C2781275326 @default.
- W2247398408 hasConcept C523546767 @default.
- W2247398408 hasConcept C54355233 @default.
- W2247398408 hasConcept C86803240 @default.
- W2247398408 hasConcept C89423630 @default.
- W2247398408 hasConceptScore W2247398408C153568844 @default.
- W2247398408 hasConceptScore W2247398408C185592680 @default.
- W2247398408 hasConceptScore W2247398408C2776154503 @default.
- W2247398408 hasConceptScore W2247398408C2781275326 @default.
- W2247398408 hasConceptScore W2247398408C523546767 @default.
- W2247398408 hasConceptScore W2247398408C54355233 @default.
- W2247398408 hasConceptScore W2247398408C86803240 @default.
- W2247398408 hasConceptScore W2247398408C89423630 @default.
- W2247398408 hasIssue "31" @default.
- W2247398408 hasLocation W22473984081 @default.
- W2247398408 hasLocation W22473984082 @default.
- W2247398408 hasOpenAccess W2247398408 @default.
- W2247398408 hasPrimaryLocation W22473984081 @default.
- W2247398408 hasRelatedWork W1649029483 @default.
- W2247398408 hasRelatedWork W1944192690 @default.
- W2247398408 hasRelatedWork W2000613781 @default.
- W2247398408 hasRelatedWork W2008946511 @default.