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• W2247444116 abstract "Stem cells are cells with capacity for self-renewal and differentiation. These cells can be isolated from all tissues of adult organisms, expanded in culture and derived in multiple lineages. Mesenchymal cells occupy a prominent position among adult stem cells. They are multipotent cells that can differentiate into osteoblasts, adipocytes, chondrocytes, neurons and hepatocytes. Mesenchymal stem cells derived from umbilical cord are functionally similar to those derived from bone marrow (classical source). Umbilical cord is an attractive alternative to bone marrow as source of mesenchymal stem cells because umbilical cord is normally discarded after birth, and the procedure for its collection is non-invasive. Currently, cell therapy represents a great hope in regenerative medicine because of the prospect of repairing damaged tissues or even the creation of the entire organs of the body. Stem cell research is still at a stage where many scientific issues need to be solved before their applications become permanent. There are numerous methods for stem cells isolation depending on both the origin of the tissue used, and the laboratories procedures. In this work we examined the spontaneous migration method to obtain these cells from Wharton's jelly of human umbilical cords. All the experiments were performed according to protocol approved by the Federal University of Pernambuco Ethics in Research Committee (No .420/2007). Using flow cytometry (FACSCalibur), we found that 70-80% of the primary cells (third passage) are positive for markers of mesenchymal cells. Application of positive selection (column-free magnetic sorting) enhanced the presence of CD90, CD44, CD29 cells to more than 90%. Such cells don’t possess the hematopoietic markers (CD45, CD34 and CD31). The doubling time of the obtained stem cells was 2-3 days. They had fibroblastoid morphology and held pronounced differentiation potential. The osteogenic differentiation was achieved by the medium supplemented with ascorbic acid, dexamethasone and β-glycerophosphate for three weeks. The differentiation was confirmed by histochemical staining (von Kossa), the presence of hydroxyapatite crystals in extracellular matrix was observed. Thereby, we can conclude that the spontaneous migration is a relatively simple method, which requires less processing time and results in a considerable number of cells with typical stem cell markers already in the primary population. The positive selection with magnetic particles is a fast and effective method to obtain highly purified stem cells." @default.
• W2247444116 created "2016-06-24" @default.
• W2247444116 creator A5006885015 @default.
• W2247444116 date "2010-01-31" @default.
• W2247444116 modified "2023-09-26" @default.
• W2247444116 title "Isolamento, expansão e diferenciação osteogênica de células-tronco mesenquimais obtidas de cordão umbilical humano" @default.
• W2247444116 cites W3190385209 @default.
• W2247444116 hasPublicationYear "2010" @default.
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