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- W2247464036 abstract "Abstract We evaluated six deproteinizing methods for determination of uric acid in serum by high-performance liquid chromatography with ultraviolet detection: those involving zinc hydroxide, sodium tungstate, trichloroacetic acid, perchloric acid, acetonitrile, and centrifugal ultrafiltration (with Amicon MPS-1 devices). We used a Toyosoda ODS-120A reversed-phase column. The mobile phase was sodium phosphate buffer (40 mmol/L, pH 2.2) containing 20 mL of methanol per liter. Absorbance of the eluate was monitored at 284 nm. The precipitation method with perchloric acid gave high recoveries of uric acid and good precision, and results agreed with those by the uricase-catalase method of Kageyama (Clin Chim Acta 1971;31:421-6)." @default.
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- W2247464036 date "1987-08-01" @default.
- W2247464036 modified "2023-10-16" @default.
- W2247464036 title "Deproteinizing methods evaluated for determination of uric acid in serum by reversed-phase liquid chromatography with ultraviolet detection." @default.
- W2247464036 doi "https://doi.org/10.1093/clinchem/33.8.1427" @default.
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