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- W2247494344 abstract "One of the many advantages that Supercritical fluid chromatography (SFC) is attributed with over high performance liquid chromatography (HPLC) is that it should be possible to use longer columns and/or columns packed with smaller particles at higher velocities. This is due to the higher diffusivity of analytes in supercritical fluids compared to liquids (higher optimum mobile phase velocity) and to the lower viscosity of the mobile phases in SFC compared to HPLC (resulting in lower pressure drops over the column). In this work the isopycnic method to construct kinetic plots for SFC was used to investigate the performance limits of an SFC system when using sub- 2 µm fully porous particles and sub- 3 µm superficially porous particles. This isopycnic kinetic plot method for SFC was developed and tested earlier for SFC separations on bare silica with pure CO2 as mobile phase. In this work, C18 columns were used in combination with more realistic modifier amounts in the mobile phase in order to show the possibilities of measuring the performance of an SFC system as a function of flow rate for realistic chromatographic experimental conditions. Kinetic plot predictions were made for separations on 1 µm and 0.5 µm particles in order to examine the possibilities of working with these very small particles on the used SFC and HPLC systems." @default.
- W2247494344 created "2016-06-24" @default.
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- W2247494344 date "2013-01-01" @default.
- W2247494344 modified "2023-10-04" @default.
- W2247494344 title "Application of the isopycnic kinetic plot method for elucidating the potential of sub-2 micron and core/shell particles in SFC" @default.
- W2247494344 hasPublicationYear "2013" @default.
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