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- W2247514908 abstract "A recombinant d-lyxose isomerase from Providencia stuartii was immobilized on the of various types beads. Beads allowed the highest conversion of fructose to mannose among the various immobilization beads evaluated and then were selected for the production of mannose from fructose. The maximum activities of both the free and immobilized enzymes for fructose isomerization were observed at pH 7.5 and 45°C in the presence of 1 mM Mn2+, and enzyme half-lives were 14 and 30 h at 35°C and 3.4 and 5.1 h at 45°C, respectively. The immobilized enzyme, in a solution of 300 g/l fructose (replaced hourly), produced 75 g/l mannose at 35°C representing a conversion yield of 25% (w/w) and a productivity of 75 g l–1 h–1 mannose after 23 cycles." @default.
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- W2247514908 date "2010-04-01" @default.
- W2247514908 modified "2023-09-27" @default.
- W2247514908 title "Application of free and immobilized D-lyxose isomerases from Providencia stuartii to the production of mannose from fructose" @default.
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