FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2247667980> ?p ?o ?g. }

• W2247667980 endingPage "380" @default.
• W2247667980 startingPage "373" @default.
• W2247667980 abstract "CCL22 inactivation in vivo occurs by cleavage at the N-terminus; however, it is unclear whether this encompasses the entire site of CCR4 interaction. CCL17 also binds CCR4 and its function requires binding via two discrete binding sites. Using monoclonal antibodies (MAbs), we report that there are two separate sites on CCL22 that are required for CCR4-mediated function. The CCL22-specific antibodies bind with affinities of 632 ± 297 pM (MC2B7) and 308 ± 43 pM (MAB4391) and neither exhibited detectable binding to CCL17. Both antibodies are comparable in their ability to inhibit CCL22-mediated calcium mobilization; however, competition binding studies demonstrate that MC2B7 and MAB4391 bind to distinct epitopes on CCL22. Both antibodies inhibit function through CCR4, which is demonstrated by loss of β-arrestin recruitment in a reporter cell line. In both assays, blocking either site independently abolished CCL22 function, suggesting that concurrent engagement of both sites with CCR4 is necessary for function. This is the first demonstration that CCL22 has two distinct binding sites that are required for CCR4 function. These antibodies are valuable tools for better understanding the interaction and function of CCL22 and CCR4 and will potentially help further understanding of the differential outcomes of CCL17 and CCL22 interaction with CCR4." @default.
• W2247667980 created "2016-06-24" @default.
• W2247667980 creator A5000047038 @default.
• W2247667980 creator A5015924450 @default.
• W2247667980 creator A5045362108 @default.
• W2247667980 creator A5070211256 @default.
• W2247667980 creator A5072492656 @default.
• W2247667980 creator A5079303599 @default.
• W2247667980 creator A5084269628 @default.
• W2247667980 date "2015-12-01" @default.
• W2247667980 modified "2023-09-24" @default.
• W2247667980 title "CCL22-specific Antibodies Reveal That Engagement of Two Distinct Binding Domains on CCL22 Is Required for CCR4-mediated Function" @default.
• W2247667980 cites W1486868171 @default.
• W2247667980 cites W1524773295 @default.
• W2247667980 cites W1532107353 @default.
• W2247667980 cites W1544336740 @default.
• W2247667980 cites W1550733776 @default.
• W2247667980 cites W1555370688 @default.
• W2247667980 cites W1857019503 @default.
• W2247667980 cites W1978385715 @default.
• W2247667980 cites W1982805461 @default.
• W2247667980 cites W1986991315 @default.
• W2247667980 cites W1987569008 @default.
• W2247667980 cites W1993504891 @default.
• W2247667980 cites W2001487688 @default.
• W2247667980 cites W2010801138 @default.
• W2247667980 cites W2013704234 @default.
• W2247667980 cites W2030836327 @default.
• W2247667980 cites W2038610464 @default.
• W2247667980 cites W2047924033 @default.
• W2247667980 cites W2078873071 @default.
• W2247667980 cites W2086411755 @default.
• W2247667980 cites W2100989466 @default.
• W2247667980 cites W2106784810 @default.
• W2247667980 cites W2107580687 @default.
• W2247667980 cites W2108452978 @default.
• W2247667980 cites W2114056456 @default.
• W2247667980 cites W2116576993 @default.
• W2247667980 cites W2117891653 @default.
• W2247667980 cites W2129741568 @default.
• W2247667980 cites W2149059793 @default.
• W2247667980 cites W2150678612 @default.
• W2247667980 cites W2154685907 @default.
• W2247667980 cites W2157329817 @default.
• W2247667980 cites W2158672984 @default.
• W2247667980 cites W2160417595 @default.
• W2247667980 cites W2170737790 @default.
• W2247667980 cites W4247055279 @default.
• W2247667980 cites W2079969065 @default.
• W2247667980 doi "https://doi.org/10.1089/mab.2015.0039" @default.
• W2247667980 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26683175" @default.
• W2247667980 hasPublicationYear "2015" @default.
• W2247667980 type Work @default.
• W2247667980 sameAs 2247667980 @default.
• W2247667980 citedByCount "3" @default.
• W2247667980 countsByYear W22476679802017 @default.
• W2247667980 countsByYear W22476679802020 @default.
• W2247667980 countsByYear W22476679802022 @default.
• W2247667980 crossrefType "journal-article" @default.
• W2247667980 hasAuthorship W2247667980A5000047038 @default.
• W2247667980 hasAuthorship W2247667980A5015924450 @default.
• W2247667980 hasAuthorship W2247667980A5045362108 @default.
• W2247667980 hasAuthorship W2247667980A5070211256 @default.
• W2247667980 hasAuthorship W2247667980A5072492656 @default.
• W2247667980 hasAuthorship W2247667980A5079303599 @default.
• W2247667980 hasAuthorship W2247667980A5084269628 @default.
• W2247667980 hasBestOaLocation W22476679801 @default.
• W2247667980 hasConcept C107824862 @default.
• W2247667980 hasConcept C12823836 @default.
• W2247667980 hasConcept C13373296 @default.
• W2247667980 hasConcept C134678942 @default.
• W2247667980 hasConcept C14036430 @default.
• W2247667980 hasConcept C143172330 @default.
• W2247667980 hasConcept C153911025 @default.
• W2247667980 hasConcept C159654299 @default.
• W2247667980 hasConcept C170493617 @default.
• W2247667980 hasConcept C185592680 @default.
• W2247667980 hasConcept C195616568 @default.
• W2247667980 hasConcept C203014093 @default.
• W2247667980 hasConcept C2775866117 @default.
• W2247667980 hasConcept C542903549 @default.
• W2247667980 hasConcept C55493867 @default.
• W2247667980 hasConcept C86803240 @default.
• W2247667980 hasConcept C95444343 @default.
• W2247667980 hasConceptScore W2247667980C107824862 @default.
• W2247667980 hasConceptScore W2247667980C12823836 @default.
• W2247667980 hasConceptScore W2247667980C13373296 @default.
• W2247667980 hasConceptScore W2247667980C134678942 @default.
• W2247667980 hasConceptScore W2247667980C14036430 @default.
• W2247667980 hasConceptScore W2247667980C143172330 @default.
• W2247667980 hasConceptScore W2247667980C153911025 @default.
• W2247667980 hasConceptScore W2247667980C159654299 @default.
• W2247667980 hasConceptScore W2247667980C170493617 @default.
• W2247667980 hasConceptScore W2247667980C185592680 @default.
• W2247667980 hasConceptScore W2247667980C195616568 @default.
• W2247667980 hasConceptScore W2247667980C203014093 @default.
• W2247667980 hasConceptScore W2247667980C2775866117 @default.
• W2247667980 hasConceptScore W2247667980C542903549 @default.

• next