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- W224775548 abstract "目的 构建包含鼠细胞转录因子白介素6 (NF-IL6)反义基因的部分功能区的重组反义真核表达载体pcDNA3.0/ NF-IL6,为进一步研究鼠巨噬细胞中NF-II6的功能奠定基础.方法 Trizol试剂法提取体外分离培养的鼠巨噬细胞总RNA,经逆转录聚合酶链式反应(RT-PCR)扩增NF-IL6基因目的 片段,PCR产物及真核表达载体pcDNA3.0分别双酶切并进行连接,转化入大肠杆菌Ecoli Dh5α扩增后获得重组载体.结果 RT-PCR获得预期大小的特异性NF-IL6DNA片段,经PCR、双酶切及测序分析证实已将鼠NF-1L6 cDNA片段正确的反向插入真核表达载体中.结论 成功获得反义pcDNA3.0/ NF- IL6重组质粒." @default.
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- W224775548 date "2011-07-05" @default.
- W224775548 modified "2023-09-23" @default.
- W224775548 title "鼠NF～IL～6反义真核表达载体的构建和鉴定" @default.
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