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- W2247759660 abstract "Caves in Mizoram, Northeast India are potential hotspot diversity regions due to the historical significance of the formation of Indo-Burman plateau and also because of their unexplored and unknown diversity. High throughput paired end illumina sequencing of V4 region of 16S rRNA was performed to systematically evaluate the bacterial community of three caves situated in Champhai district of Mizoram, Northeast India. A total of 10,643 operational taxonomic units (based on 97% cutoff) comprising 21 bacterial phyla and 21 candidate phyla with a sequencing depth of 11, 40013 were found in this study. The overall taxonomic profile obtained by BLAST against RDP classifier and Greengene OTU database revealed high diversity within the bacterial communities, dominated by Planctomycetes, Actinobacteria, Proteobacteria, Bacteroidetes, and Firmicutes, while members of archea were less diverse and mainly comprising of eukaryoarchea. Analysis revealed that Farpuk (CFP) cave has low diversity and is mainly dominated by actinobacteria (80% reads), whereas diverse communities were found in the caves of Murapuk (CMP) and Lamsialpuk (CLP). Analysis of rare and abundant species also revealed that a major portion of the identified OTUs were falling under rare biosphere. Significantly, all these caves recorded a high number of unclassified OUTs which might represent novel species. Further, analysis with whole genome sequencing is needed to validate the novel species as well as to determine their functional significance." @default.
- W2247759660 created "2016-06-24" @default.
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- W2247759660 date "2014-11-22" @default.
- W2247759660 modified "2023-09-26" @default.
- W2247759660 title "Metagenomic analysis of bacterial community composition among the cave sediments of Indo-Burman biodiversity hotspot region" @default.
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- W2247759660 doi "https://doi.org/10.7287/peerj.preprints.631v1" @default.
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