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- W2248404972 abstract "The demand for tissue engineered articular cartilage for implantation in patients withosteoarthritis requires the development of stable and robust process production systems.We have developed a shear stress and perfusion bioreactor (SSPB) for tissue engineeringcartilage to enhance nutrient diffusion and mechanical stimuli. Using static and bioreactorculture in 3D scaffolds we have identified tissue culture conditions using transforminggrowth factor beta-1 (TGF -1), bone morphogenic protein-2 (BMP-2) and micromasscell seeding of hMSCs in polycaprolactone (PCL) scaffolds. hMSCs were seeded onporous PCL scaffolds using the following conditions: no grow factors, TGF -1 or BMP-2alone, TGF -1+BMP-2 simultaneously and transient application of BMP-2 in thepresence of TGF -1. We found that the transient use of BMP-2 in the presence of TGF -1 greatly enhances differentiation of hMSCs as shown by GAG deposition and collagenII expression. We tested this condition in the rotating disc bioreactor as a proof ofconcept. We found an increase in cell proliferation and extra cellular matrix production inthe bioreactor compared to static culture.%%%%M.S.%%%%1 online resource (xii, 113 p.) : ill. (some col.), facsim.%%%%%%%%Biomedical Engineering%%%%Engineering%%%%Bioreactor Tissue engineering Cartilage%%%%UNRESTRICTED" @default.
- W2248404972 created "2016-06-24" @default.
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- W2248404972 date "2010-01-01" @default.
- W2248404972 modified "2023-09-27" @default.
- W2248404972 title "Expansion and differentiation of human mesenchymal stem cells in a perfusion and surface shear stress bioreactor for engineered tissue cartilage" @default.
- W2248404972 hasPublicationYear "2010" @default.
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