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- W2250227530 abstract "This chapter discusses the methods of preparation of Dopamine β-Hydroxylase (Beef Adrenal). One unit of dopamine β-hydroxylase is defined as that amount which catalyzes the formation of 1 micromole of norepinephrine per minute per milliliter of reaction mixture under conditions of the assay. Specific activity is units/milligram of protein. The fluorometric method is not applicable to crude tissue preparations because of the presence of endogenous catechols, which interfere with the determination of norepinephrine. Many other assay methods have been developed to overcome this problem. These include a periodate procedure in which dopamine labeled with 14 C in the terminal position of the side chain is used as the substrate. The norepinephrine formed is treated with periodate to liberate radioactive formaldehyde which is then trapped as the dimedon precipitate and assayed for radioactivity. A second complication of assaying the enzyme in crude tissue preparations is the presence of inhibitors of the enzyme in crude extracts. These inhibitors are removed during the early stages of the purification procedure resulting in a large increase in total activity." @default.
- W2250227530 created "2016-06-24" @default.
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- W2250227530 date "1971-01-01" @default.
- W2250227530 modified "2023-09-27" @default.
- W2250227530 title "[242] Dopamine β-hydroxylase (beef adrenal)" @default.
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- W2250227530 doi "https://doi.org/10.1016/0076-6879(71)17131-5" @default.
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