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• W2250452187 abstract "Objective To investigate effects of clenbuterol (CLB) on the peroxisome proliferators-activated receptor γ (PPARγ) expression in adipose tissues of rats. Methods Twenty adult female Sprague-Dawley rats were randomly divided into 4 groups (5 rats per group). CLB solved in normal saline solution was given at the dose of 0 mg/kg body weight (bw) (group A, as the control), 0.4 mg/kg bw (group B, low-dose group), 2.0 mg/kg bw (group C, mid-dose group), and 18.5 mg/kg bw (group D, high-dose group) for 14 d by gavage consecutively, respectively. Methods of immunohistochemistry, quantitative Real-time PCR and Western blotting were performed to detect expression of PPARγ in the adipose tissue samples. Results PPARγ-positive immunostaining was strong in the controls and weak in the experimental groups. There was no difference on PPARγ mRNA and protein between the low-dose group and the control (P>0.05). With the increase of CLB doses, expression levels of PPARγ mRNA and protein were significantly lower in mid- or high-dose group than those in the control (P Conclusions The PPARγ expression in adipose tissues of rats could be down-regulated after CLB exposure, and the decrease became more severe with the increasing doses." @default.
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• W2250452187 date "2015-09-01" @default.
• W2250452187 modified "2023-09-22" @default.
• W2250452187 title "Effects of clenbuterol exposure on PPARγ expression in adipocytes of rats" @default.
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• W2250452187 doi "https://doi.org/10.7669/j.issn.1001-7844.2015.02.0067" @default.
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