Matches in SemOpenAlex for { <https://semopenalex.org/work/W2250461275> ?p ?o ?g. }
- W2250461275 abstract "CUNHA, G.G.S. Peroxidative activity of enzymatic extracts from Ceriporiopsis subvermispora and its correlation with lignin degradation. 2008. 66 f. Dissertation (Master of Science in Industrial Biotechnology) Escola de Engenharia de Lorena, Universidade de Sao Paulo, 2008. The white-rot fungi are able to degrade lignocellulosic materials in an efficient way through reactions catalyzed by hydrolytic and oxidative enzymes or still by means of low molar mass metabolites that begin the degradative process. Ceriporiopsis subvermispora is a white-rot fungus that stands out as one of the most selective in lignin degradation, possessing great potential for technological applications. This fungus is able to initiate lipid peroxidation reactions based on the Mn produced by the catalytic action of the enzyme manganese peroxidase (MnP) on Mn ions. In the present study, the secretion of oxidative enzymes by C. subvermispora was evaluated in cultures containing Eucalyptus grandis wood chips and some supplements such as corn steep liquor and corn steep liquor plus glucose. The enzymatic extracts obtained from these cultures contained high MnP activity, mainly when corn steep liquor was used as a culture supplement. The same extracts were able to peroxidize linoleic acid in vitro. In these reactions, thiobarbituric acid reactive substances (TBARS) accumulated over reaction time and the TBARS levels were proportional to the MnP present in the extracts. The rate of oxygen consumption in these reactions was also proportional to the level of MnP present in the extracts. The characterization of the biotreated wood indicated that the largest lignin and wood mass losses were observed in supplemented cultures, with an apparent correlation between the peroxidative activity in the extracts and the extension of the wood and lignin degradation. Laccase was detected only in low levels in the enzymatic extracts originating from the wood-containing cultures. On the other hand, submerged cultures in which the liquid medium was composed of 2% malt-extract and 0,2% yeast extract induced laccase production without simultaneous secretion of MnP. The laccase active extract was also able to peroxidize linoleic acid, since in the presence of a mediator (ρ-hydroxy-benzoic acid). Anyway, during E. grandis biodegradation by C. subvermispora, laccase was only detected in the beginning of the cultivations and even so at low levels suggesting that laccase were not involved in lipid peroxidation during the E. grandis wood biodegradation by C. subvermispora. KEY WOODS: Ceriporiopsis subvermispora. Manganese Peroxidase. Lipid peroxidation. LISTA DE FIGURAS Figura 1: Estrutura-modelo da lignina proposta por Adler 13 Figura 2: Sistema de camadas na parede das celulas de madeira 14 Figura 3: Ciclo catalitico simplificado de peroxidase dependente de Manganes (MnP) ....... 18 Figura 4: Ciclo catalitico de lacase 18 Figura 5: Peroxidacao de lipideos insaturados iniciada por Mn ou radical hidroxila 21 Figura 6: Degradacao de um composto modelo de lignina (nao fenolico) pelo sistema MnP, acido linoleico e H2O2 24 Figura 7: Producao de lacases por C. subvermispora durante a biodegradacao de E. grandis 44 Figura 8: Producao de MnP por C. subvermispora durante a biodegradacao de E. grandis.. 45 Figura 9: Consumo de O2 em reacoes de peroxidacao de lipideos catalisadas por extratos enzimaticos obtidos de E. grandis biotratado por C. subvermispora 46 Figura 10: Relacao entre os niveis de MnP em extratos de cultivos de C. subvermispora sobre E. grandis e as taxas de consumo de O2 em reacoes de peroxidacao de acido linoleico catalisadas por esses mesmos extratos 48 Figura 11: TBARS acumulados apos 1 h de reacao catalisada por extratos obtidos de cultivos de C. subvermispora sobre E. grandis 49 Figura 12: Relacao entre o consumo de oxigenio e o acumulo de TBARS em reacoes de peroxidacao de acido linoleico catalisadas por extratos de cultivos de C. subvermispora sobre E. grandis 50 Figura 13: Quantidade residual de acido linoleico e hexanal formado em funcao do tempo de reacao de acido linoleico com um extrato contendo elevada atividade de MnP 52 Figura 14: Concentracao de TBARS na madeira e variacao de pH dos cultivos de C. subvermispora sobre E. grandis 53 Figura 15: Perfil metabolico de C. subvermispora ao longo de 14 dias de cultivo em meio liquido composto de 2% de extrato de malte e 0,2% de extrato de levedura ........ 55 Figura 16: Consumo de O2 em reacoes de peroxidacao de lipideos catalisadas por extratos enzimaticos com alta atividade de lacase obtidos em cultivos liquidos de E. grandis biotratado por C. subvermispora 57 Figura 17: Relacao entre o consumo de oxigenio e o tempo de biodegradacao em cultivos liquidos de C. subvermispora em meio liquido composto de extrato de malte (2,4%) e extrato de levedura (0,7%) 57 Figura 18: Relacao entre os niveis de Lacase em filtrados de cultivos de C. subvermispora em meio liquido e as taxas de consumo de O2 em reacoes de peroxidacao de acido linoleico 58 LISTA DE TABELAS Tabela 1: Condicoes de cultivo de C. subvermispora SS-3 sobre madeira E. grandis utilizando um sistema de fermentacao em meio solido 32 Tabela 2: Perda de massa e componentes da madeira E. grandis biotratado por 28 dias com C. subvermispora em diferentes condicoes de cultivo 42" @default.
- W2250461275 created "2016-06-24" @default.
- W2250461275 creator A5083900222 @default.
- W2250461275 date "2014-01-01" @default.
- W2250461275 modified "2023-09-26" @default.
- W2250461275 title "UNIVERSIDADE DE SÃO PAULO ESCOLA DE ENGENHARIA DE LORENA" @default.
- W2250461275 cites W10344325 @default.
- W2250461275 cites W126109032 @default.
- W2250461275 cites W1273527354 @default.
- W2250461275 cites W1276934726 @default.
- W2250461275 cites W134803944 @default.
- W2250461275 cites W1419950595 @default.
- W2250461275 cites W1485283179 @default.
- W2250461275 cites W1485300768 @default.
- W2250461275 cites W1489047317 @default.
- W2250461275 cites W1489485264 @default.
- W2250461275 cites W1492932443 @default.
- W2250461275 cites W1496418103 @default.
- W2250461275 cites W1498849689 @default.
- W2250461275 cites W1510774684 @default.
- W2250461275 cites W1517204576 @default.
- W2250461275 cites W1524166571 @default.
- W2250461275 cites W1524961740 @default.
- W2250461275 cites W1526439584 @default.
- W2250461275 cites W1533554324 @default.
- W2250461275 cites W1535358083 @default.
- W2250461275 cites W1536322421 @default.
- W2250461275 cites W1536837298 @default.
- W2250461275 cites W1546685326 @default.
- W2250461275 cites W1548811000 @default.
- W2250461275 cites W1550259820 @default.
- W2250461275 cites W1558786924 @default.
- W2250461275 cites W1561076003 @default.
- W2250461275 cites W1564206460 @default.
- W2250461275 cites W1565843248 @default.
- W2250461275 cites W1568067817 @default.
- W2250461275 cites W1570309408 @default.
- W2250461275 cites W1572248281 @default.
- W2250461275 cites W1576783369 @default.
- W2250461275 cites W1579160354 @default.
- W2250461275 cites W1584664119 @default.
- W2250461275 cites W1587088742 @default.
- W2250461275 cites W1589356041 @default.
- W2250461275 cites W1590932878 @default.
- W2250461275 cites W1603328630 @default.
- W2250461275 cites W1604636323 @default.
- W2250461275 cites W1605058418 @default.
- W2250461275 cites W1606391478 @default.
- W2250461275 cites W1607271942 @default.
- W2250461275 cites W1664000111 @default.
- W2250461275 cites W1676101252 @default.
- W2250461275 cites W1808803353 @default.
- W2250461275 cites W1846547318 @default.
- W2250461275 cites W1890321828 @default.
- W2250461275 cites W1908637113 @default.
- W2250461275 cites W1937684822 @default.
- W2250461275 cites W1964380519 @default.
- W2250461275 cites W1964879532 @default.
- W2250461275 cites W1965194542 @default.
- W2250461275 cites W1965327998 @default.
- W2250461275 cites W1965355553 @default.
- W2250461275 cites W1965472166 @default.
- W2250461275 cites W1965817992 @default.
- W2250461275 cites W1966940129 @default.
- W2250461275 cites W1967034374 @default.
- W2250461275 cites W1967045660 @default.
- W2250461275 cites W1968834637 @default.
- W2250461275 cites W1969870516 @default.
- W2250461275 cites W1970449891 @default.
- W2250461275 cites W1970746755 @default.
- W2250461275 cites W1970810015 @default.
- W2250461275 cites W1972634787 @default.
- W2250461275 cites W1973029229 @default.
- W2250461275 cites W1976039477 @default.
- W2250461275 cites W1976423215 @default.
- W2250461275 cites W1976740214 @default.
- W2250461275 cites W1977165056 @default.
- W2250461275 cites W1977724400 @default.
- W2250461275 cites W1977916228 @default.
- W2250461275 cites W1978170396 @default.
- W2250461275 cites W1978234893 @default.
- W2250461275 cites W1978399588 @default.
- W2250461275 cites W1978733918 @default.
- W2250461275 cites W1978898803 @default.
- W2250461275 cites W1980857424 @default.
- W2250461275 cites W1982243180 @default.
- W2250461275 cites W1982374584 @default.
- W2250461275 cites W1982535196 @default.
- W2250461275 cites W1984008313 @default.
- W2250461275 cites W1984570526 @default.
- W2250461275 cites W1985252740 @default.
- W2250461275 cites W1985750874 @default.
- W2250461275 cites W1986537971 @default.
- W2250461275 cites W1987325925 @default.
- W2250461275 cites W1987467679 @default.
- W2250461275 cites W1988102906 @default.
- W2250461275 cites W1988503331 @default.
- W2250461275 cites W1989147665 @default.
- W2250461275 cites W1989341100 @default.
- W2250461275 cites W1989559567 @default.