FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2252364906> ?p ?o ?g. }

• W2252364906 endingPage "679" @default.
• W2252364906 startingPage "679" @default.
• W2252364906 abstract "679 Background: While determination of HER-2 status in breast cancer by fluorescence in situ hybridization (FISH) is now widely employed, its utility as part of a QC/QA program for IHC has not been established. The aim of this study was to (a) determine whether the use of FISH could improve the utility of immunohistochemistry (IHC), (b) attempt to identify sources of discrepancy between HER-2 protein, tested by IHC and HER-2 gene status, tested by FISH, and (c) design a reasonable and cost-effective testing algorithm. Methods: We commenced a QA/QC program for HER-2 testing in 1999, accruing 3564 tumors to date. As part of the program, parallel IHC testing was performed for each clinical FISH test. Routine components of this program also include random monthly review of HER-2 IHC and FISH results, targeted review of tumors with discrepant results, and intra- and inter-pathologist review of IHC and FISH scores. FISH and IHC tests were performed using FDA-approved testing kits (Vysis/Abbott and DakoCytomation, respectively). Results: With FISH to be the presumed gold standard, the sensitivity of IHC was 92.6%. The specificity of positive (3+) overexpression was 98%. The positive and negative predictive values of IHC were 94.8% and 97.4% respectively. Using some of the components of this QA/QC program, we were able to identify tumors with potential IHC-FISH discrepancy. These include tumors with (a) heterogeneity of gene status, and (b) polysomy of chromosome 17 (with/without true gene amplification). Significant discordance was only observed in the 2+ IHC group. Conclusions: Establishing a strict QC/QA program allowed us to significantly improve the sensitivity and specificity of IHC testing. According to these results, IHC can be used for screening. FISH testing should be mandatory only on tumors with 2+ IHC scores. Such program also helped identify sources of discrepant IHC and FISH results. Tumors containing either instable HER-2/neu gene or polysomy of chromosome 17 are largely the source of discrepant IHC and FISH results, and should be further investigated. Author Disclosure Employment or Leadership Consultant or Advisory Stock Ownership Honoraria Research Funding Expert Testimony Other Remuneration NexCura NexCura Genentech, Inc." @default.
• W2252364906 created "2016-06-24" @default.
• W2252364906 creator A5024477555 @default.
• W2252364906 creator A5042471676 @default.
• W2252364906 creator A5065349135 @default.
• W2252364906 creator A5071098811 @default.
• W2252364906 creator A5080534060 @default.
• W2252364906 creator A5081900680 @default.
• W2252364906 date "2004-07-15" @default.
• W2252364906 modified "2023-09-27" @default.
• W2252364906 title "Discrepancies between immunohistochemistry and fluorescence in situ hybridization for HER-2 testing in breast cancer: The role of a quality assurance program on a cohort of 3564 patients" @default.
• W2252364906 doi "https://doi.org/10.1200/jco.2004.22.14_suppl.679" @default.
• W2252364906 hasPublicationYear "2004" @default.
• W2252364906 type Work @default.
• W2252364906 sameAs 2252364906 @default.
• W2252364906 citedByCount "2" @default.
• W2252364906 crossrefType "journal-article" @default.
• W2252364906 hasAuthorship W2252364906A5024477555 @default.
• W2252364906 hasAuthorship W2252364906A5042471676 @default.
• W2252364906 hasAuthorship W2252364906A5065349135 @default.
• W2252364906 hasAuthorship W2252364906A5071098811 @default.
• W2252364906 hasAuthorship W2252364906A5080534060 @default.
• W2252364906 hasAuthorship W2252364906A5081900680 @default.
• W2252364906 hasConcept C104317684 @default.
• W2252364906 hasConcept C121608353 @default.
• W2252364906 hasConcept C126322002 @default.
• W2252364906 hasConcept C142724271 @default.
• W2252364906 hasConcept C143998085 @default.
• W2252364906 hasConcept C204232928 @default.
• W2252364906 hasConcept C2777542201 @default.
• W2252364906 hasConcept C2781303241 @default.
• W2252364906 hasConcept C2909208804 @default.
• W2252364906 hasConcept C3018290483 @default.
• W2252364906 hasConcept C30481170 @default.
• W2252364906 hasConcept C505870484 @default.
• W2252364906 hasConcept C530470458 @default.
• W2252364906 hasConcept C54355233 @default.
• W2252364906 hasConcept C71924100 @default.
• W2252364906 hasConcept C86803240 @default.
• W2252364906 hasConceptScore W2252364906C104317684 @default.
• W2252364906 hasConceptScore W2252364906C121608353 @default.
• W2252364906 hasConceptScore W2252364906C126322002 @default.
• W2252364906 hasConceptScore W2252364906C142724271 @default.
• W2252364906 hasConceptScore W2252364906C143998085 @default.
• W2252364906 hasConceptScore W2252364906C204232928 @default.
• W2252364906 hasConceptScore W2252364906C2777542201 @default.
• W2252364906 hasConceptScore W2252364906C2781303241 @default.
• W2252364906 hasConceptScore W2252364906C2909208804 @default.
• W2252364906 hasConceptScore W2252364906C3018290483 @default.
• W2252364906 hasConceptScore W2252364906C30481170 @default.
• W2252364906 hasConceptScore W2252364906C505870484 @default.
• W2252364906 hasConceptScore W2252364906C530470458 @default.
• W2252364906 hasConceptScore W2252364906C54355233 @default.
• W2252364906 hasConceptScore W2252364906C71924100 @default.
• W2252364906 hasConceptScore W2252364906C86803240 @default.
• W2252364906 hasIssue "14_suppl" @default.
• W2252364906 hasLocation W22523649061 @default.
• W2252364906 hasOpenAccess W2252364906 @default.
• W2252364906 hasPrimaryLocation W22523649061 @default.
• W2252364906 hasRelatedWork W1964082899 @default.
• W2252364906 hasRelatedWork W1979531350 @default.
• W2252364906 hasRelatedWork W2032786655 @default.
• W2252364906 hasRelatedWork W2043504824 @default.
• W2252364906 hasRelatedWork W2134347179 @default.
• W2252364906 hasRelatedWork W2161942325 @default.
• W2252364906 hasRelatedWork W2323384383 @default.
• W2252364906 hasRelatedWork W2386381782 @default.
• W2252364906 hasRelatedWork W2408907530 @default.
• W2252364906 hasRelatedWork W4324117074 @default.
• W2252364906 hasVolume "22" @default.
• W2252364906 isParatext "false" @default.
• W2252364906 isRetracted "false" @default.
• W2252364906 magId "2252364906" @default.
• W2252364906 workType "article" @default.