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- W2252482464 abstract "The Citrus were used to extract protein and optimized by the TCA-acedone,Tris-HCl extraction and improved method of PBS,and then after SDS-PAGE,mass spectrometry and Western blot.The result showed protein concentration is 30 μg/μL by the improved of PBS extraction methods.The SDS-PAGE result presented the bright and clear bands,with low background and could be identified by mass spectrometry.Furthemore,by applying transgenic pthA-nls gene in sweet orange plants,the PthA protein which is related to the Xanthomonas axonopodis pv.citri.could be found.To be concluded,the pthA-nls gene has been transferred into sweet orange and the expressive protein produced immunoreaction." @default.
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- W2252482464 date "2010-06-30" @default.
- W2252482464 modified "2023-09-26" @default.
- W2252482464 title "Extraction of <I>Citrus</I> leaf protein and analysis of Western blot of the <I>Citrus</I> canker protein PthA" @default.
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- W2252482464 doi "https://doi.org/10.3724/sp.j.1238.2010.00300" @default.
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