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- W2253079897 abstract "In this work the synthetic sequence in length 194 nucleotide pairs is constructed. (DNA-matrix). The analysis of the sequence in GenBank by means of program BLAST has shown that oligonucleotide sequence haven’t homologues. One of key components of reaction are «primers» synthetic oligonucleotides. The length of amplificated fragment is defined by distance between primers. At amplification with polymerase chain reaction two oligonucleotide primers are used. Theoretical aspects of designing primers are considered Two primers in the sizes 20 nucleotide pairs were synthesized to DNA-matrix. By means of program Primer3 it is picked temperature of fusion (Тm) for corresponding primers: for right primer Тm = 59,80°С, for left primer Тm = 60,25°С. The optimum temperature of a corresponding mode in the program amplification (annealing temperature) is: for left primer ta = 56,00°С, for right primer ta = 55,80°С." @default.
- W2253079897 created "2016-06-24" @default.
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- W2253079897 date "2012-01-01" @default.
- W2253079897 modified "2023-09-23" @default.
- W2253079897 title "Основные аспекты конструирования праймеров для определения видовой принадлежности ДНК крупного рогатого скота методом полимеразной цепной реакции" @default.
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