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- W2253769235 abstract "We investigated the effects of genistein in mammary gland carcinogenesis by incoporation of 2H from ²H₂O into the deoxyribose (dR) moiety of purine deoxyribonucleotides in dividing cells. Female Sprague?Dawley rats were fed estrogen free AIN-93G diet starting on 1 week before breeding and continuing through pregnancy and lactation. Female pups were assigned to the following groups on postnatal day 16 and fed AIN-93G diet: vehicle (dimethylsulfoxide) (DMSO); genistein; estradiol benzoate(EB). On postnatal days 16, 18 and 20, female pups were injected subcutaneously with 250 μg genistein/g body wt, 500 ng EB/g body wt or an equivalent volume of the vehicle. At day 50 postpartum, half of each group were gavaged with 60mg dimethylbenz[a]anthracene(DMBA) in perila oil. After 1 week of DMBA treatment, all animals were labeled with ²H₂O by administration of 4% ²H₂O in drinking water after single intraperitonial bolus injection with 99.9% ²H₂O until sacrifice on postnatal day 81. The timedependent weight gains were observed in all groups throughout the experimental period. The enrichment of body ²H₂O was attained at 1.84-2.47% through oral administration of ²H₂O. Mammary epithelial cell proliferation was measured by enrichment (EM1) of dA from rats. DMBA treated groups showed higher fractional synthesis than DMBA non-treated groups. The group exposed only to genistein showed significantly lower EM1 (1.46±0.87%) than those of control groups, i.e. DMBA non-treated group (2.28±0.29%) and DMBA treated group(2.32±0.28%). Bromodeoxyuridine (BrdU) immunostaining of mammary tissue revealed that genistein reduced proliferation of the mammary epithelial, and the number of cells stained positive for BrdU both in DMBA treated groups and DMBA non-treated groups. H&E staining of mammary epithelium also showed that the exposure to genistein decreased proliferation of the mammary epithelium. The epithelium in the rats treated with DMBA showed mostly multiple cell layers, in contrast to the mostly double layer shown in the DMBA non-treated rats. In conclusion, exposure to genistein in the prepubertal period inhibited mammary epithelial cell proliferation. The ²H₂O labeling results were in good agreement with the results of BrdU incorporation and histomorphometry." @default.
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- W2253769235 date "2005-07-01" @default.
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- W2253769235 title "The Role of Genistein in Mammary Gland Carcinogenesis: A New Approach Using Stable Isotope(²H₂O)-Mass Spectrometry Technique" @default.
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