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- W2254158308 abstract "Abstract Thienodolin (THN) features a tricyclic indole‐ S‐ hetero scaffold that encompasses two unique carbon–sulfur bonds. Although its biosynthetic gene cluster has been recently identified in Streptomyces albogriseolus , the essential enzymes for the formation of C−S bonds have been relatively unexplored. Here, we isolated and characterized a new biosynthetic gene cluster from Streptomyces sp. FXJ1.172. Heterologous expression, systematic gene inactivation, and in vitro biochemical characterization enable us to determine the minimum set of genes for THN synthesis, and an aminotransferase (ThnJ) for catalyzing the downstream conversion of tryptophan chlorination. In addition, we evaluated (and mainly excluded) a previously assumed pivotal intermediate by feeding experiments. With these results, we narrowed down four enzymes (ThnC–F) that are responsible for the two unprecedented C−S bond formations. Our study provides a solid basis for further unraveling of the unique C−S mechanisms." @default.
- W2254158308 created "2016-06-24" @default.
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- W2254158308 date "2016-03-21" @default.
- W2254158308 modified "2023-10-16" @default.
- W2254158308 title "Identifying the Minimal Enzymes for Unusual Carbon-Sulfur Bond Formation in Thienodolin Biosynthesis" @default.
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- W2254158308 doi "https://doi.org/10.1002/cbic.201500670" @default.
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