Matches in SemOpenAlex for { <https://semopenalex.org/work/W2254943896> ?p ?o ?g. }
Showing items 1 to 96 of
96
with 100 items per page.
- W2254943896 endingPage "413" @default.
- W2254943896 startingPage "409" @default.
- W2254943896 abstract "Objective To study the expression and mutation of β-catenin in non-small cell lung cancer (NSCLC) and the relationships between expression, mutation and clinicopathological parameters and prognosis. Methods All the 120 samples (fixed with 10% formalin and embedded in paraffin blocks) were obtained from patients with NSCLC, who underwent surgery in the First Affiliated Hospital of China Medical University from 2001 to 2002 and Anshan Tumor Hospital from 1980 to 2001. Follow-up was available in 67 patients. The 53 fresh NSCLC samples and corresponding normal lung tissues were obtained from the First Affiliated Hospital of China Medical University. The pattern of β-catenin protein expression was detected by immunohistochemistry method (120 samples) using anti-β-catenin antibody (1∶600). The level of β-catenin protein expression was detected by Western Blotting method using anti-β-catenin antibody (1∶1500) in 53 fresh NSCLC samples and corresponding normal lung tissues. The mutation of β-catenin gene exon 3 was detected by polymerase chain reaction (PCR) and direct sequencing method (53 samples). Results In 120 patients, abnormal expression rate of β-catenin was 80% (96/120) and nuclear expression rate was 36.7% (44/120). There was significant difference in β-catenin expression between well differentiated and poorly differentiated NSCLC. In well and moderately differentiated NSCLC cells the abnormal expression rate was 69.2% (45/65), which was much lower than 92.7% (51/55) of poorly differentiated ones (P=0.003). β-catenin expressed abnormally in 86.5% (64/74) cases with lymph node metastasis and in 69.6% (32/46) cases without lymph node metastasis (P=0.044). The results of Western Blotting showed that the expression of β-catenin in NSCLC tissues was significantly higher than that in normal lung tissues (t=2.935, P=0.005). The 53 patients’ DNA were analysed by PCR and direct sequencing method, but no mutation in β-catenin gene exon 3 was found. Conclusion Abnormal expression of β-catenin is negatively associated with differentiation and positively associated with lymph node metastasis of NSCLC. The β-catenin gene exon 3 mutation is not the main reason of β-catenin abnormal expression in NSCLC.DOI: 10.3779/j.issn.1009-3419.2004.05.08" @default.
- W2254943896 created "2016-06-24" @default.
- W2254943896 creator A5023811312 @default.
- W2254943896 creator A5030967805 @default.
- W2254943896 creator A5073511506 @default.
- W2254943896 creator A5086609279 @default.
- W2254943896 creator A5086664647 @default.
- W2254943896 date "2004-10-20" @default.
- W2254943896 modified "2023-09-27" @default.
- W2254943896 title "[Expression and mutation of β-catenin in non-small cell lung cancer]." @default.
- W2254943896 doi "https://doi.org/10.3779/j.issn.1009-3419.2004.05.08" @default.
- W2254943896 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21244793" @default.
- W2254943896 hasPublicationYear "2004" @default.
- W2254943896 type Work @default.
- W2254943896 sameAs 2254943896 @default.
- W2254943896 citedByCount "5" @default.
- W2254943896 countsByYear W22549438962019 @default.
- W2254943896 countsByYear W22549438962021 @default.
- W2254943896 crossrefType "journal-article" @default.
- W2254943896 hasAuthorship W2254943896A5023811312 @default.
- W2254943896 hasAuthorship W2254943896A5030967805 @default.
- W2254943896 hasAuthorship W2254943896A5073511506 @default.
- W2254943896 hasAuthorship W2254943896A5086609279 @default.
- W2254943896 hasAuthorship W2254943896A5086664647 @default.
- W2254943896 hasConcept C104317684 @default.
- W2254943896 hasConcept C126322002 @default.
- W2254943896 hasConcept C137620995 @default.
- W2254943896 hasConcept C142724271 @default.
- W2254943896 hasConcept C159654299 @default.
- W2254943896 hasConcept C203014093 @default.
- W2254943896 hasConcept C204232928 @default.
- W2254943896 hasConcept C2776256026 @default.
- W2254943896 hasConcept C2777714996 @default.
- W2254943896 hasConcept C2780849966 @default.
- W2254943896 hasConcept C2994225774 @default.
- W2254943896 hasConcept C30145163 @default.
- W2254943896 hasConcept C36823959 @default.
- W2254943896 hasConcept C49105822 @default.
- W2254943896 hasConcept C501734568 @default.
- W2254943896 hasConcept C502942594 @default.
- W2254943896 hasConcept C55493867 @default.
- W2254943896 hasConcept C71924100 @default.
- W2254943896 hasConcept C86803240 @default.
- W2254943896 hasConcept C98638677 @default.
- W2254943896 hasConceptScore W2254943896C104317684 @default.
- W2254943896 hasConceptScore W2254943896C126322002 @default.
- W2254943896 hasConceptScore W2254943896C137620995 @default.
- W2254943896 hasConceptScore W2254943896C142724271 @default.
- W2254943896 hasConceptScore W2254943896C159654299 @default.
- W2254943896 hasConceptScore W2254943896C203014093 @default.
- W2254943896 hasConceptScore W2254943896C204232928 @default.
- W2254943896 hasConceptScore W2254943896C2776256026 @default.
- W2254943896 hasConceptScore W2254943896C2777714996 @default.
- W2254943896 hasConceptScore W2254943896C2780849966 @default.
- W2254943896 hasConceptScore W2254943896C2994225774 @default.
- W2254943896 hasConceptScore W2254943896C30145163 @default.
- W2254943896 hasConceptScore W2254943896C36823959 @default.
- W2254943896 hasConceptScore W2254943896C49105822 @default.
- W2254943896 hasConceptScore W2254943896C501734568 @default.
- W2254943896 hasConceptScore W2254943896C502942594 @default.
- W2254943896 hasConceptScore W2254943896C55493867 @default.
- W2254943896 hasConceptScore W2254943896C71924100 @default.
- W2254943896 hasConceptScore W2254943896C86803240 @default.
- W2254943896 hasConceptScore W2254943896C98638677 @default.
- W2254943896 hasIssue "5" @default.
- W2254943896 hasLocation W22549438961 @default.
- W2254943896 hasLocation W22549438962 @default.
- W2254943896 hasOpenAccess W2254943896 @default.
- W2254943896 hasPrimaryLocation W22549438961 @default.
- W2254943896 hasRelatedWork W2330951032 @default.
- W2254943896 hasRelatedWork W2350093130 @default.
- W2254943896 hasRelatedWork W2352727692 @default.
- W2254943896 hasRelatedWork W2357610286 @default.
- W2254943896 hasRelatedWork W2358217791 @default.
- W2254943896 hasRelatedWork W2359683392 @default.
- W2254943896 hasRelatedWork W2365081796 @default.
- W2254943896 hasRelatedWork W2369391784 @default.
- W2254943896 hasRelatedWork W2373511072 @default.
- W2254943896 hasRelatedWork W2374488415 @default.
- W2254943896 hasRelatedWork W2376908134 @default.
- W2254943896 hasRelatedWork W2381881657 @default.
- W2254943896 hasRelatedWork W2385280617 @default.
- W2254943896 hasRelatedWork W2389531896 @default.
- W2254943896 hasRelatedWork W2902276810 @default.
- W2254943896 hasRelatedWork W3028109006 @default.
- W2254943896 hasRelatedWork W3030062820 @default.
- W2254943896 hasRelatedWork W3031886531 @default.
- W2254943896 hasRelatedWork W3048884695 @default.
- W2254943896 hasRelatedWork W3201255201 @default.
- W2254943896 hasVolume "7" @default.
- W2254943896 isParatext "false" @default.
- W2254943896 isRetracted "false" @default.
- W2254943896 magId "2254943896" @default.
- W2254943896 workType "article" @default.