Matches in SemOpenAlex for { <https://semopenalex.org/work/W2255102893> ?p ?o ?g. }
- W2255102893 endingPage "979" @default.
- W2255102893 startingPage "970" @default.
- W2255102893 abstract "HD-GYPs make up a subclass of the metal-dependent HD phosphohydrolase superfamily and catalyze conversion of cyclic di(3′,5′)-guanosine monophosphate (c-di-GMP) to 5′-phosphoguanylyl-(3′→5′)-guanosine (pGpG) and GMP. Until now, the only reported crystal structure of an HD-GYP that also exhibits c-di-GMP phosphodiesterase activity contains a His/carboxylate ligated triiron active site. However, other structural and phylogenetic correlations indicate that some HD-GYPs contain dimetal active sites. Here we provide evidence that an HD-GYP c-di-GMP phosphodiesterase, TM0186, from Thermotoga maritima can accommodate both di- and trimetal active sites. We show that an as-isolated iron-containing TM0186 has an oxo/carboxylato-bridged diferric site, and that the reduced (diferrous) form is necessary and sufficient to catalyze conversion of c-di-GMP to pGpG, but that conversion of pGpG to GMP requires more than two metals per active site. Similar c-di-GMP phosphodiesterase activities were obtained with divalent iron or manganese. On the basis of activity correlations with several putative metal ligand residue variants and molecular dynamics simulations, we propose that TM0186 can accommodate both di- and trimetal active sites. Our results also suggest that a Glu residue conserved in a subset of HD-GYPs is required for formation of the trimetal site and can also serve as a labile ligand to the dimetal site. Given the anaerobic growth requirement of T. maritima, we suggest that this HD-GYP can function in vivo with either divalent iron or manganese occupying di- and trimetal sites." @default.
- W2255102893 created "2016-06-24" @default.
- W2255102893 creator A5053901330 @default.
- W2255102893 creator A5055112988 @default.
- W2255102893 date "2016-02-01" @default.
- W2255102893 modified "2023-09-26" @default.
- W2255102893 title "Active Site Metal Occupancy and Cyclic Di-GMP Phosphodiesterase Activity of <i>Thermotoga maritima</i> HD-GYP" @default.
- W2255102893 cites W1523229642 @default.
- W2255102893 cites W1890914571 @default.
- W2255102893 cites W1977304935 @default.
- W2255102893 cites W1998379819 @default.
- W2255102893 cites W2014723793 @default.
- W2255102893 cites W2025347123 @default.
- W2255102893 cites W2025628802 @default.
- W2255102893 cites W2026161233 @default.
- W2255102893 cites W2029667189 @default.
- W2255102893 cites W2056503383 @default.
- W2255102893 cites W2069358906 @default.
- W2255102893 cites W2069651362 @default.
- W2255102893 cites W2077883858 @default.
- W2255102893 cites W2084919208 @default.
- W2255102893 cites W2086323657 @default.
- W2255102893 cites W2093832152 @default.
- W2255102893 cites W2097167157 @default.
- W2255102893 cites W2099430193 @default.
- W2255102893 cites W2113420364 @default.
- W2255102893 cites W2113557404 @default.
- W2255102893 cites W2117548490 @default.
- W2255102893 cites W2132665880 @default.
- W2255102893 cites W2132759034 @default.
- W2255102893 cites W2143108902 @default.
- W2255102893 cites W2150981663 @default.
- W2255102893 cites W2156062569 @default.
- W2255102893 cites W2265414501 @default.
- W2255102893 cites W2266428948 @default.
- W2255102893 cites W2314199588 @default.
- W2255102893 doi "https://doi.org/10.1021/acs.biochem.5b01227" @default.
- W2255102893 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5055813" @default.
- W2255102893 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26786892" @default.
- W2255102893 hasPublicationYear "2016" @default.
- W2255102893 type Work @default.
- W2255102893 sameAs 2255102893 @default.
- W2255102893 citedByCount "17" @default.
- W2255102893 countsByYear W22551028932016 @default.
- W2255102893 countsByYear W22551028932017 @default.
- W2255102893 countsByYear W22551028932018 @default.
- W2255102893 countsByYear W22551028932019 @default.
- W2255102893 countsByYear W22551028932020 @default.
- W2255102893 countsByYear W22551028932021 @default.
- W2255102893 countsByYear W22551028932022 @default.
- W2255102893 crossrefType "journal-article" @default.
- W2255102893 hasAuthorship W2255102893A5053901330 @default.
- W2255102893 hasAuthorship W2255102893A5055112988 @default.
- W2255102893 hasBestOaLocation W22551028932 @default.
- W2255102893 hasConcept C104317684 @default.
- W2255102893 hasConcept C116569031 @default.
- W2255102893 hasConcept C170493617 @default.
- W2255102893 hasConcept C178790620 @default.
- W2255102893 hasConcept C181199279 @default.
- W2255102893 hasConcept C185592680 @default.
- W2255102893 hasConcept C2776111186 @default.
- W2255102893 hasConcept C2777002520 @default.
- W2255102893 hasConcept C2777995097 @default.
- W2255102893 hasConcept C41183919 @default.
- W2255102893 hasConcept C512185932 @default.
- W2255102893 hasConcept C547475151 @default.
- W2255102893 hasConcept C55493867 @default.
- W2255102893 hasConcept C62826618 @default.
- W2255102893 hasConcept C71240020 @default.
- W2255102893 hasConcept C74884574 @default.
- W2255102893 hasConceptScore W2255102893C104317684 @default.
- W2255102893 hasConceptScore W2255102893C116569031 @default.
- W2255102893 hasConceptScore W2255102893C170493617 @default.
- W2255102893 hasConceptScore W2255102893C178790620 @default.
- W2255102893 hasConceptScore W2255102893C181199279 @default.
- W2255102893 hasConceptScore W2255102893C185592680 @default.
- W2255102893 hasConceptScore W2255102893C2776111186 @default.
- W2255102893 hasConceptScore W2255102893C2777002520 @default.
- W2255102893 hasConceptScore W2255102893C2777995097 @default.
- W2255102893 hasConceptScore W2255102893C41183919 @default.
- W2255102893 hasConceptScore W2255102893C512185932 @default.
- W2255102893 hasConceptScore W2255102893C547475151 @default.
- W2255102893 hasConceptScore W2255102893C55493867 @default.
- W2255102893 hasConceptScore W2255102893C62826618 @default.
- W2255102893 hasConceptScore W2255102893C71240020 @default.
- W2255102893 hasConceptScore W2255102893C74884574 @default.
- W2255102893 hasFunder F4320337354 @default.
- W2255102893 hasIssue "6" @default.
- W2255102893 hasLocation W22551028931 @default.
- W2255102893 hasLocation W22551028932 @default.
- W2255102893 hasLocation W22551028933 @default.
- W2255102893 hasLocation W22551028934 @default.
- W2255102893 hasOpenAccess W2255102893 @default.
- W2255102893 hasPrimaryLocation W22551028931 @default.
- W2255102893 hasRelatedWork W1608795484 @default.
- W2255102893 hasRelatedWork W1983382530 @default.
- W2255102893 hasRelatedWork W1999441690 @default.
- W2255102893 hasRelatedWork W2026904289 @default.