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• W2255353486 abstract "Hybridoma technology has long been a remarkable and indispensable platform for generating high-quality monoclonal antibodies (mAbs). Hybridoma-derived mAbs have not only served as powerful tool reagents but also have emerged as the most rapidly expanding class of therapeutic biologics. With the establishment of mAb humanization and with the development of transgenic-humanized mice, hybridoma technology has opened new avenues for effectively generating humanized or fully human mAbs as therapeutics. In this chapter, an overview of hybridoma technology and the laboratory procedures used routinely for hybridoma generation are discussed and detailed in the following sections: cell fusion for hybridoma generation, antibody screening and characterization, hybridoma subcloning and mAb isotyping, as well as production of mAbs from hybridoma cells." @default.
• W2255353486 created "2016-06-24" @default.
• W2255353486 creator A5068920468 @default.
• W2255353486 date "2012-01-01" @default.
• W2255353486 modified "2023-10-14" @default.
• W2255353486 title "Hybridoma Technology for the Generation of Monoclonal Antibodies" @default.
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• W2255353486 doi "https://doi.org/10.1007/978-1-61779-931-0_7" @default.
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