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- W2255407103 abstract "ABSTRACT BACKGROUND: We have recently developed a method for labeling and following the migration of keratocytes using the fluorescent dye, 46diamidino-2-phenylindole (DAPI), which intercalates specifically into the adenine-thymine base pairs of deoxyribonucleic acid (DNA). METHODS: DAPI was applied to a 2-millimeter area of the corneal stroma, at the edge of the microkeratome wound, prior to placement of the autograft. RESULTS: DAPI-labeled keratocytes were identiby epifluorescence microscopy along the microkeratome wound and in the mid-periphery of the lamellar graft by day 7. In contrast, no DAPI-labeled keratocytes were observed in the graft immediately following surgery, indicating the DAPI-labeled keratocytes observed in the graft at day 7 resulted from keratocyte migration, and not from diffusion of DAPI from the host into the graft. CONCLUSIONS: These investigations suggest that DAPI could be a useful probe for monitoring the migration of keratocytes into corneal grafts following lamellar keratoplasty and other corneal transplant surgical techniques. [Refract Corneal Surg 1992;8:230-234.)" @default.
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- W2255407103 date "1992-05-01" @default.
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- W2255407103 title "An In-Vivo Technique for Monitoring Keratocyte Migration Following Lamellar Keratoplasty" @default.
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- W2255407103 doi "https://doi.org/10.3928/1081-597x-19920501-10" @default.
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