Matches in SemOpenAlex for { <https://semopenalex.org/work/W2255864537> ?p ?o ?g. }
Showing items 1 to 75 of
75
with 100 items per page.
- W2255864537 abstract "Small Molecule Inhibitors of the SARS-CoV Nsp15 Endoribonuclease, Mechanism of Action and Insight into Coronavirus Infection. (May 2009) Joanna Maria Ortiz Alcantara, B.S., National Autonomous University of Mexico Chair of Advisory Committee: Dr. Cheng C. Kao The Severe Acute Respiratory Syndrome (SARS) virus encodes several unusual RNA processing enzymes, including Nsp15, an endoribonuclease that preferentially cleaves 3’ of uridylates through a Ribonuclease A-like mechanism. Crystal structures of Nsp15 confirmed that the Nsp15 active site is structurally similar to that of Ribonuclease A. These similarities and our molecular docking analysis lead us to hypothesize that previously characterized Ribonuclease A inhibitors will also inhibit the SARS-CoV Nsp15. Benzopurpurin B, C-467929, C-473872, N-36711, N-65828, N-103018 and Congo red were tested for effects on Nsp15 endoribonuclease activity. A real-time fluorescence assay revealed that the IC50 values for inhibiting Nsp15 were between 0.2 μM and 40 μM. Benzopurpurin B, C-473872, and Congo red are competitive inhibitors, according to kinetic studies and were demonstrated to bind SARS-CoV Nsp15 by a differential scanning fluorimetry assay. Benzopurpurin B also inhibited the Nsp15 orthologs from two other coronaviruses: mouse hepatitis virus (MHV) and infectious bronchitis virus. The three compounds reduced infectivity of MHV in L2 cells by 8 to 26 fold. The more effective drugs also caused a decrease in MHV RNA accumulation." @default.
- W2255864537 created "2016-06-24" @default.
- W2255864537 creator A5014888099 @default.
- W2255864537 creator A5024598682 @default.
- W2255864537 date "2010-07-14" @default.
- W2255864537 modified "2023-09-27" @default.
- W2255864537 title "SMALL MOLECULE INHIBITORS OF THE SARS-COV NSP15 ENDORIBONUCLEASE, MECHANISM OF ACTION AND INSIGHT INTO CORONAVIRUS INFECTION" @default.
- W2255864537 hasPublicationYear "2010" @default.
- W2255864537 type Work @default.
- W2255864537 sameAs 2255864537 @default.
- W2255864537 citedByCount "0" @default.
- W2255864537 crossrefType "book" @default.
- W2255864537 hasAuthorship W2255864537A5014888099 @default.
- W2255864537 hasAuthorship W2255864537A5024598682 @default.
- W2255864537 hasConcept C104317684 @default.
- W2255864537 hasConcept C10879258 @default.
- W2255864537 hasConcept C142724271 @default.
- W2255864537 hasConcept C153911025 @default.
- W2255864537 hasConcept C159047783 @default.
- W2255864537 hasConcept C181199279 @default.
- W2255864537 hasConcept C185592680 @default.
- W2255864537 hasConcept C2777648638 @default.
- W2255864537 hasConcept C2778976237 @default.
- W2255864537 hasConcept C2779134260 @default.
- W2255864537 hasConcept C2780102198 @default.
- W2255864537 hasConcept C3008058167 @default.
- W2255864537 hasConcept C524204448 @default.
- W2255864537 hasConcept C55493867 @default.
- W2255864537 hasConcept C67705224 @default.
- W2255864537 hasConcept C71924100 @default.
- W2255864537 hasConcept C86803240 @default.
- W2255864537 hasConceptScore W2255864537C104317684 @default.
- W2255864537 hasConceptScore W2255864537C10879258 @default.
- W2255864537 hasConceptScore W2255864537C142724271 @default.
- W2255864537 hasConceptScore W2255864537C153911025 @default.
- W2255864537 hasConceptScore W2255864537C159047783 @default.
- W2255864537 hasConceptScore W2255864537C181199279 @default.
- W2255864537 hasConceptScore W2255864537C185592680 @default.
- W2255864537 hasConceptScore W2255864537C2777648638 @default.
- W2255864537 hasConceptScore W2255864537C2778976237 @default.
- W2255864537 hasConceptScore W2255864537C2779134260 @default.
- W2255864537 hasConceptScore W2255864537C2780102198 @default.
- W2255864537 hasConceptScore W2255864537C3008058167 @default.
- W2255864537 hasConceptScore W2255864537C524204448 @default.
- W2255864537 hasConceptScore W2255864537C55493867 @default.
- W2255864537 hasConceptScore W2255864537C67705224 @default.
- W2255864537 hasConceptScore W2255864537C71924100 @default.
- W2255864537 hasConceptScore W2255864537C86803240 @default.
- W2255864537 hasLocation W22558645371 @default.
- W2255864537 hasOpenAccess W2255864537 @default.
- W2255864537 hasPrimaryLocation W22558645371 @default.
- W2255864537 hasRelatedWork W2051534962 @default.
- W2255864537 hasRelatedWork W2738380970 @default.
- W2255864537 hasRelatedWork W2888130829 @default.
- W2255864537 hasRelatedWork W2952783687 @default.
- W2255864537 hasRelatedWork W3013515371 @default.
- W2255864537 hasRelatedWork W3025955122 @default.
- W2255864537 hasRelatedWork W3037112981 @default.
- W2255864537 hasRelatedWork W3047457453 @default.
- W2255864537 hasRelatedWork W3084440171 @default.
- W2255864537 hasRelatedWork W3091637359 @default.
- W2255864537 hasRelatedWork W3094854996 @default.
- W2255864537 hasRelatedWork W3111006228 @default.
- W2255864537 hasRelatedWork W3126082010 @default.
- W2255864537 hasRelatedWork W3134653186 @default.
- W2255864537 hasRelatedWork W3164420600 @default.
- W2255864537 hasRelatedWork W3178428800 @default.
- W2255864537 hasRelatedWork W3190773265 @default.
- W2255864537 hasRelatedWork W3199341326 @default.
- W2255864537 hasRelatedWork W3210697779 @default.
- W2255864537 hasRelatedWork W2551493595 @default.
- W2255864537 isParatext "false" @default.
- W2255864537 isRetracted "false" @default.
- W2255864537 magId "2255864537" @default.
- W2255864537 workType "book" @default.