FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2256280841> ?p ?o ?g. }

• W2256280841 abstract "Over the last years, fungal diseases have become an increasing problem due to the use of broad-spectrum prophylaxes, chemotherapy and transplantation. Moreover, immunocompromised population (e.g., acquired immunodeficiency syndrome patients) is more likely than healthy one to develop fungal infections such as candidiasis. This caused a change in the spectrum of life-threatening pathogens, with Candida species such as Candida dubliniensis becoming increasingly important in medical microbiology. A recognized virulence trait of Candida albicans, shared by Candida dubliniensis is dimorphism, i.e., the ability to change between a filamentous and a yeast form. Insights into Candida dubliniensis virulence were recently stressed in a report using an animal model and tissue culture system, where filamentation ability and virulence were connected. Even though the very close phylogenetic relationship between both Candida species, Candida dubliniensis pathogenicity mechanisms are less well known. The ability of microorganisms to sense and respond to extracellular factors has been investigated in prokaryotes. In fact, cell-cell signalling molecules were identified and shown to modulate biological functions such as virulence factors expression, biofilm formation or cell density. This has been an increasing area of research interest in eukaryotes, mainly concerning Candida albicans morphological regulation. Indeed, E,E-farnesol and tyrosol were identified as cellcell signalling molecules in Candida albicans and have been demonstrated to regulate morphogenesis. In light of these findings, some authors suggested that discrepancies in the induction and kinetics of filamentation in Candida albicans and Candida dubliniensis should be due to different signals and pathways, even though to date these have not been recognized. Aiming to contribute to a better understanding of Candida albicans and Candida dubliniensis pathogenicity, the main objectives of this study were to gain insights into morphogenesis control and biofilm development throughout chemical signals in these Candida species. In the last years several authors highlighted 3-methyl-1butanol, 2-phenylethanol, 1-dodecanol and nerolidol as potential morphogenetic regulators to be evaluated. Candida albicans CECT 1472 (Coleccion Espanola de Cultivos Tipo, Spain) and Candida dubliniensis CBS 7987 (Centraalbureau voor Schimmelcultures, Germany) standardized cells suspensions 1 × 10 yeast cells/ ml were prepared in RPMI 1640 medium. On one hand, the effect of specific alcohols in planktonic grown cells was assayed by adding such compounds to the culture medium at the final concentrations: 23 mM and 46 μM for 3-methyl-1-butanol, 500 μM and 5 μM, for 2-phenylethanol, 2 μM and 2 nM for 1-dodecanol and 150 μM, 1.5 μM and 1.5 nM for nerolidol. Yeast cells were grown at 37 ° C on a mechanical shaker at 130 rpm for 12 h. After that, Candida cells morphology was assessed by light microscopy analysis and the percentage of filamentation calcutated. Candida viability was assessed by spectrophotometric determinations. On the other hand, for biofilm experiments, Candida albicans and Candida dubliniensis biofilms were developed on the surface of microtriter plates and allowed to adhere for three hours at 37 °C, 130 rpm. To determine the effect of the specific alcohols on Candida biofilms formation and development, they were added at the beginning of the adhesion process or afterwards, respectively. The same concentrations of alcohols were used in planktonic and biofilm assays. Biofilms morphology was characterized by scanning electron microscopy. Quantitative measurements of biofilm formation were performed colorimetrically by a semiquantitative 2,3bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide reduction assay. Results obtained show that 3-methyl-1-butanol, 2-phenylethanol, 1-dodecanol and nerolidol regulate Candida albicans and Candida dubliniensis morphogenesis, repressing filamentation in a dose dependent fashion (Table 1). TABLE 1. . Effect of alcohols on the morphology of planktonic grown Candida albicans CECT 1472 and Candida dubliniensis CBS 7987. Percentages of filamentation inhibition were calculated and expressed as mean ± standard deviation. Compound Concentration Candida albicans Candida dubliniensis (% filamentation inhibition) 3-methyl-1-butanol 23 mM 95. 82 ± 2.96 92.38 ± 2.14 46 μM 68.41 ± 12.30 70.70 ± 15.61 2-phenylethanol 500 μM 95.32 ± 1.23 91.54 ± 10.66 5 μM 71.43 ± 15.75 72.95 ± 16.98 1-dodecanol 2 μM 69.85 ± 16.82 74.52 ± 9.63 2 nM 57.76 ± 14.35 54.14 ± 12.09 nerolidol 1.5 μM 64.79 ± 4.68 64.59 ± 7.81 1.5 nM 51.09 ± 9.31 68.31 ± 5.02 These compounds were noted to be active even at low concentrations (micromolar for 3-methyl-1-butanol and 2-phenylethanol and nanomolar 1-dodecanol and nerolidol). Moreover, it was observed that similar percentages of inhibition were obtained for both Candida species, suggesting that Candida albicans and Candida dubliniensis may share mechanisms of signalling and response to these alcohols. The significance of these compounds in biofilm formation and development will be discussed. These studies will contribute to a better understanding of the morphogenesis and biofilm regulation networks in Candida albicans and Candida dubliniensis throughout specific alcohols signalling." @default.
• W2256280841 created "2016-06-24" @default.
• W2256280841 creator A5001958608 @default.
• W2256280841 creator A5008068226 @default.
• W2256280841 creator A5029481092 @default.
• W2256280841 creator A5060670302 @default.
• W2256280841 date "2008-01-01" @default.
• W2256280841 modified "2023-09-24" @default.
• W2256280841 title "The role of alcohol compounds in Candida albicans and Candida dubliniensis morphogenesis and biofilm development" @default.
• W2256280841 hasPublicationYear "2008" @default.
• W2256280841 type Work @default.
• W2256280841 sameAs 2256280841 @default.
• W2256280841 citedByCount "0" @default.
• W2256280841 crossrefType "journal-article" @default.
• W2256280841 hasAuthorship W2256280841A5001958608 @default.
• W2256280841 hasAuthorship W2256280841A5008068226 @default.
• W2256280841 hasAuthorship W2256280841A5029481092 @default.
• W2256280841 hasAuthorship W2256280841A5060670302 @default.
• W2256280841 hasConcept C104317684 @default.
• W2256280841 hasConcept C118687296 @default.
• W2256280841 hasConcept C145081991 @default.
• W2256280841 hasConcept C2776445003 @default.
• W2256280841 hasConcept C2779443200 @default.
• W2256280841 hasConcept C2780917455 @default.
• W2256280841 hasConcept C2908647359 @default.
• W2256280841 hasConcept C523546767 @default.
• W2256280841 hasConcept C54355233 @default.
• W2256280841 hasConcept C58123911 @default.
• W2256280841 hasConcept C60987743 @default.
• W2256280841 hasConcept C71924100 @default.
• W2256280841 hasConcept C86803240 @default.
• W2256280841 hasConcept C89423630 @default.
• W2256280841 hasConcept C99454951 @default.
• W2256280841 hasConceptScore W2256280841C104317684 @default.
• W2256280841 hasConceptScore W2256280841C118687296 @default.
• W2256280841 hasConceptScore W2256280841C145081991 @default.
• W2256280841 hasConceptScore W2256280841C2776445003 @default.
• W2256280841 hasConceptScore W2256280841C2779443200 @default.
• W2256280841 hasConceptScore W2256280841C2780917455 @default.
• W2256280841 hasConceptScore W2256280841C2908647359 @default.
• W2256280841 hasConceptScore W2256280841C523546767 @default.
• W2256280841 hasConceptScore W2256280841C54355233 @default.
• W2256280841 hasConceptScore W2256280841C58123911 @default.
• W2256280841 hasConceptScore W2256280841C60987743 @default.
• W2256280841 hasConceptScore W2256280841C71924100 @default.
• W2256280841 hasConceptScore W2256280841C86803240 @default.
• W2256280841 hasConceptScore W2256280841C89423630 @default.
• W2256280841 hasConceptScore W2256280841C99454951 @default.
• W2256280841 hasLocation W22562808411 @default.
• W2256280841 hasOpenAccess W2256280841 @default.
• W2256280841 hasPrimaryLocation W22562808411 @default.
• W2256280841 hasRelatedWork W123245757 @default.
• W2256280841 hasRelatedWork W158820662 @default.
• W2256280841 hasRelatedWork W16263325 @default.
• W2256280841 hasRelatedWork W165761898 @default.
• W2256280841 hasRelatedWork W1982476880 @default.
• W2256280841 hasRelatedWork W2132956775 @default.
• W2256280841 hasRelatedWork W2145307402 @default.
• W2256280841 hasRelatedWork W2171281278 @default.
• W2256280841 hasRelatedWork W2184333680 @default.
• W2256280841 hasRelatedWork W2266447039 @default.
• W2256280841 hasRelatedWork W2320435488 @default.
• W2256280841 hasRelatedWork W2498487781 @default.
• W2256280841 hasRelatedWork W2750118287 @default.
• W2256280841 hasRelatedWork W2791065914 @default.
• W2256280841 hasRelatedWork W2961194336 @default.
• W2256280841 hasRelatedWork W2990217600 @default.
• W2256280841 hasRelatedWork W3029880179 @default.
• W2256280841 hasRelatedWork W3130431128 @default.
• W2256280841 hasRelatedWork W3214629028 @default.
• W2256280841 hasRelatedWork W68080148 @default.
• W2256280841 isParatext "false" @default.
• W2256280841 isRetracted "false" @default.
• W2256280841 magId "2256280841" @default.
• W2256280841 workType "article" @default.