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- W2256978385 abstract "The possibility of transferring genes to lentil (Lens culinaris Medik.) cotyledonary nodes via accelerated and DNA-coated tungsten particles was investigated. Cotyledonary nodes isolated from a local lentil cultivar (Sultan-1) were utilized as targets for bombardment. DNA in the form of circular plasmid (pBSGUSINT) was precipitated on tungsten particles (ca. 2 mm diameter) by the calcium-nitrate method. Cotyledonary nodes were bombarded by an electronic processor controlled GeneboosterTM particle delivery instrument driven by compressed nitrogen gas. Bombardment was carried out at varying gas pressure and chamber vacuum. Bombarded material was subjected to histochemical GUS staining 24 hours after shooting. Almost 50 percent of the bombarded material expressed the transferred GUS gene as deduced by the blue color formation on the cotyledonary nodes. Negative controls (non-bombarded and or cotyledonary nodes bombarded only with DNA uncoated tungsten particles) did not exhibit GUS activity. Shoots regenerated from bombarded cotyledonary nodes also exhibited patches of GUS staining, indicating stable integration and expression of the transferred gene. Currently, the optimized bombardment method is being used for genetic manipulation of local lentil cultivars against herbicide resistance" @default.
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- W2256978385 date "1999-01-01" @default.
- W2256978385 modified "2023-10-18" @default.
- W2256978385 title "Gus gene delivery and expression in lentil cotyledonary nodes using particle bombardment" @default.
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