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• W2257619762 abstract "El objetivo del estudio fue optimizar la tecnica de microinjerto in vitro de apices caulinares (MIV) para eliminar Candidatus Liberibacter asiaticus (CLas) en cultivares de citricos infectados. Como fuentes de brotes se emplearon plantas de cinco cultivares con sintomas de huanglongbing (HLB): mandarinos Satsuma Tropical y Parson’s Special, los hibridos de mandarinos Sunburst IVIA 200-C-1 y tangelo Nova IVIA 74-7 y limero Persa SRA-58. Para estandarizar el tamano de apice, suficiente para la eliminacion de CLas, se ensayaron tres dimensiones compuestas por el meristemo apical con 2, 3 y 4 primordios foliares (pf) (0,1 - 0,4 mm). La presencia de CLas se constato mediante PCR duplex en las plantas sintomaticas y PCR anidada en las plantas obtenidas por MIV. Se alcanzo un prendimiento general de 71,79%, de ellos el 46,15% para apices de 2 pf y superior al 80% para 3 y 4 pf. De los microinjertos logrados, mas del 60% crecio y este parametro estuvo por encima del 40% para apices de 2 y 3 pf; mientras que el 80,61% correspondio a apices de 4 pf. El 100% de las plantas obtenidas, a partir de los microinjertos, se mantuvieron libres de CLas por cuatro anos, independientemente del tamano del apice utilizado. Los resultados confirmaron la efectividad de la tecnica de microinjerto in vitro para sanear cultivares de citricos infectados por CLas. Palabras clave: microinjerto in vitro, saneamiento, huanglongbing, PCR, nPCR. Use of shoot-tip grafting in vitro to eliminate ´Candidatus Liberibacter asiaticus´ in citrus cultivars in Cuba ABSTRACT: The aim of this research was to optimize the in vitro shoot-tip grafting (STG) technique to eliminate Candidatus Liberibacter asiaticus (CLas) from Citrus infected cultivars. Five citrus cultivars (Satsuma Tropical and Parson’s Special mandarins, Sunburst IVIA 200-C-1 and Nova IVIA 74-7 hybrids of mandarins and Persian SRA-58 lime) showing symptoms of huanglongbing (HLB) were used as bud source:. Three different apex sizes, composed of the apical meristem with two, three and four leaf primordia (lp) (0.1 - 0.4 mm), were tested to standardize the apex size required to eliminate CLas. The presence of the pathogen was verified by the conventional PCR in the symptomatic plants and the nested PCR in the asymptomatic ones. The final success percentage of the shoot-tip grafting was 71.79%; 46.15% for the apex with 2 lp and over 80% for apexes with 3 and 4 lp. Sixty per cent of all the shoot-tip grafting grew normally, and this parameter was over 40% when apexes with 2 and 3 lpwere used, whereas it reached 80.61% when the apex with 4 lp was used. The 100% of the shoot-tip grafted plants were diagnosed as CLas free and this status was kept for four years regardless the shoot-tip size used. The results confirmed the reliability of the STG for removing CLas from citrus infected cultivars. Key words: shoot-tip grafting, sanitation, huanglongbing, PCR, nPCR." @default.
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• W2257619762 date "2015-08-07" @default.
• W2257619762 modified "2023-09-24" @default.
• W2257619762 title "Uso del microinjerto in vitro de ápices caulinares para eliminar ´Candidatus Liberibacter asiaticus´ en cultivares de cítricos en Cuba" @default.
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