Matches in SemOpenAlex for { <https://semopenalex.org/work/W2258272015> ?p ?o ?g. }
- W2258272015 endingPage "442" @default.
- W2258272015 startingPage "428" @default.
- W2258272015 abstract "Post-transcriptional 3′ end addition of nucleotides is important in a variety of RNA decay pathways. We have examined the 3′ end addition of nucleotides during the decay of the Hsp70 mRNA and a corresponding reporter RNA in Drosophila S2 cells by conventional sequencing of cDNAs obtained after mRNA circularization and by deep sequencing of dedicated libraries enriched for 3′ decay intermediates along the length of the mRNA. Approximately 5%–10% of 3′ decay intermediates carried nonencoded oligo(A) tails with a mean length of 2–3 nucleotides. RNAi experiments showed that the oligoadenylated RNA fragments were intermediates of exosomal decay and the noncanonical poly(A) polymerase Trf4-1 was mainly responsible for A addition. A hot spot of A addition corresponded to an intermediate of 3′ decay that accumulated upon inhibition of decapping, and knockdown of Trf4-1 increased the abundance of this intermediate, suggesting that oligoadenylation facilitates 3′ decay. Oligoadenylated 3′ decay intermediates were found in the cytoplasmic fraction in association with ribosomes, and fluorescence microscopy revealed a cytoplasmic localization of Trf4-1. Thus, oligoadenylation enhances exosomal mRNA degradation in the cytoplasm." @default.
- W2258272015 created "2016-06-24" @default.
- W2258272015 creator A5004533161 @default.
- W2258272015 creator A5021241158 @default.
- W2258272015 creator A5030361061 @default.
- W2258272015 creator A5041558179 @default.
- W2258272015 creator A5055728996 @default.
- W2258272015 creator A5074565657 @default.
- W2258272015 date "2016-01-19" @default.
- W2258272015 modified "2023-10-17" @default.
- W2258272015 title "Oligoadenylation of 3′ decay intermediates promotes cytoplasmic mRNA degradation in <i>Drosophila</i> cells" @default.
- W2258272015 cites W1585169926 @default.
- W2258272015 cites W1893726980 @default.
- W2258272015 cites W1964628768 @default.
- W2258272015 cites W1969840430 @default.
- W2258272015 cites W1971644927 @default.
- W2258272015 cites W1979277911 @default.
- W2258272015 cites W1981442065 @default.
- W2258272015 cites W1987777159 @default.
- W2258272015 cites W1988309554 @default.
- W2258272015 cites W1991588710 @default.
- W2258272015 cites W1992169314 @default.
- W2258272015 cites W1996862303 @default.
- W2258272015 cites W2004259423 @default.
- W2258272015 cites W2008934800 @default.
- W2258272015 cites W2015722126 @default.
- W2258272015 cites W2016674854 @default.
- W2258272015 cites W2023561018 @default.
- W2258272015 cites W2031707270 @default.
- W2258272015 cites W2032501207 @default.
- W2258272015 cites W2033366350 @default.
- W2258272015 cites W2034616998 @default.
- W2258272015 cites W2034774838 @default.
- W2258272015 cites W2035886282 @default.
- W2258272015 cites W2036880206 @default.
- W2258272015 cites W2038305786 @default.
- W2258272015 cites W2039357236 @default.
- W2258272015 cites W2041877620 @default.
- W2258272015 cites W2045565555 @default.
- W2258272015 cites W2048318131 @default.
- W2258272015 cites W2048532977 @default.
- W2258272015 cites W2062012140 @default.
- W2258272015 cites W2065538503 @default.
- W2258272015 cites W2066514893 @default.
- W2258272015 cites W2066666252 @default.
- W2258272015 cites W2067058481 @default.
- W2258272015 cites W2067293798 @default.
- W2258272015 cites W2071849521 @default.
- W2258272015 cites W2072770570 @default.
- W2258272015 cites W2076627305 @default.
- W2258272015 cites W2079523547 @default.
- W2258272015 cites W2081587978 @default.
- W2258272015 cites W2085860799 @default.
- W2258272015 cites W2088399865 @default.
- W2258272015 cites W2089004878 @default.
- W2258272015 cites W2092896767 @default.
- W2258272015 cites W2093205227 @default.
- W2258272015 cites W2094089023 @default.
- W2258272015 cites W2095950646 @default.
- W2258272015 cites W2104158537 @default.
- W2258272015 cites W2104752637 @default.
- W2258272015 cites W2106601322 @default.
- W2258272015 cites W2106838809 @default.
- W2258272015 cites W2108424630 @default.
- W2258272015 cites W2117613120 @default.
- W2258272015 cites W2118134712 @default.
- W2258272015 cites W2120597581 @default.
- W2258272015 cites W2121354785 @default.
- W2258272015 cites W2122292621 @default.
- W2258272015 cites W2124985265 @default.
- W2258272015 cites W2125762953 @default.
- W2258272015 cites W2127001229 @default.
- W2258272015 cites W2127278857 @default.
- W2258272015 cites W2133163771 @default.
- W2258272015 cites W2134530067 @default.
- W2258272015 cites W2137557128 @default.
- W2258272015 cites W2137564327 @default.
- W2258272015 cites W2140406771 @default.
- W2258272015 cites W2141172277 @default.
- W2258272015 cites W2141326112 @default.
- W2258272015 cites W2142084170 @default.
- W2258272015 cites W2142398748 @default.
- W2258272015 cites W2142571092 @default.
- W2258272015 cites W2146558686 @default.
- W2258272015 cites W2147997023 @default.
- W2258272015 cites W2153700854 @default.
- W2258272015 cites W2153970661 @default.
- W2258272015 cites W2156435521 @default.
- W2258272015 cites W2158010566 @default.
- W2258272015 cites W2158266834 @default.
- W2258272015 cites W2161277720 @default.
- W2258272015 cites W2162737849 @default.
- W2258272015 cites W2163731437 @default.
- W2258272015 cites W2171469387 @default.
- W2258272015 cites W233265278 @default.
- W2258272015 cites W2887358862 @default.
- W2258272015 doi "https://doi.org/10.1261/rna.053942.115" @default.
- W2258272015 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4748820" @default.