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- W2258382904 abstract "In this study,hyaluronidase(HYL) was used as a bait protein to screen its interacted protein from the human lung cDNA library using the yeast two hybrid system to explore its function in SS2 infection.The full length Hyl gene was amplified from chromosomal DNA of SS2 strain SC21 by PCR.Hly gene was cloned directed into pSos vector to yield the bait plasmid pSos-Hyl.The recombinant expression plasmid pET-28c-Hyl was constructed by the same way at the same time.The library was screened by co-transformation of the pSos-Hyl bait constructed into a temperature-sensitive cdc25H yeast strain.By screening and testing repeatedly,two positive target plasmid was got from the human lung cDNA library.One of them was identical to Homo sapiens adducin 3 gene(ADD3).The other showed high homology to assembly ion transporter protein gene of homo sapiens(AITP).The protein-protein interaction between HYL and targets received was confirmed by ProFound Co-immunoprecipation assay.The relation between the function of ADD3 or AITP and the pathopoiesis was analyzed." @default.
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- W2258382904 date "2009-01-01" @default.
- W2258382904 modified "2023-09-25" @default.
- W2258382904 title "Screening for new binding proteins which interact with HYL of Streptocuccus suis serotype 2 using yeast two hybrid." @default.
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