Matches in SemOpenAlex for { <https://semopenalex.org/work/W2258442600> ?p ?o ?g. }
Showing items 1 to 91 of
91
with 100 items per page.
- W2258442600 endingPage "110" @default.
- W2258442600 startingPage "101" @default.
- W2258442600 abstract "A major challenge for ancient DNA (aDNA) studies using museum specimens is that sampling procedures usually involve at least the partial destruction of each specimen used, such as the removal of skin, pieces of bone, or a tooth. Recently, a nondestructive DNA extraction method was developed for the extraction of amplifiable DNA fragments from museum specimens without appreciable damage to the specimen. Here, we examine the utility of this method by attempting DNA extractions from historic (older than 70 years) chimpanzee specimens. Using this method, we PCR-amplified part of the mitochondrial HVR-I region from 65% (56/86) of the specimens from which we attempted DNA extraction. However, we found a high incidence of multiple sequences in individual samples, suggesting substantial cross-contamination among samples, most likely originating from storage and handling in the museums. Consequently, reproducible sequences could be reconstructed from only 79% (44/56) of the successfully extracted samples, even after multiple extractions and amplifications. This resulted in an overall success rate of just over half (44/86 of samples, or 51% success), from which 39 distinct HVR-I haplotypes were recovered. We found a high incidence of C to T changes, arguing for both low concentrations of and substantial damage to the endogenous DNA. This chapter highlights both the potential and the limitations of nondestructive DNA extraction from museum specimens." @default.
- W2258442600 created "2016-06-24" @default.
- W2258442600 creator A5010332653 @default.
- W2258442600 creator A5050601584 @default.
- W2258442600 creator A5051083372 @default.
- W2258442600 date "2011-12-08" @default.
- W2258442600 modified "2023-09-26" @default.
- W2258442600 title "Case Study: Using a Nondestructive DNA Extraction Method to Generate mtDNA Sequences from Historical Chimpanzee Specimens" @default.
- W2258442600 cites W1506969536 @default.
- W2258442600 cites W1849332773 @default.
- W2258442600 cites W1967549174 @default.
- W2258442600 cites W1969869762 @default.
- W2258442600 cites W1979287319 @default.
- W2258442600 cites W2013367562 @default.
- W2258442600 cites W2039785774 @default.
- W2258442600 cites W2052773191 @default.
- W2258442600 cites W2055574416 @default.
- W2258442600 cites W2061837724 @default.
- W2258442600 cites W2086619661 @default.
- W2258442600 cites W2119073635 @default.
- W2258442600 cites W2121531763 @default.
- W2258442600 cites W2125736251 @default.
- W2258442600 cites W2125797555 @default.
- W2258442600 cites W2130144437 @default.
- W2258442600 cites W2130460780 @default.
- W2258442600 cites W2139778720 @default.
- W2258442600 cites W2158714788 @default.
- W2258442600 cites W2166680137 @default.
- W2258442600 doi "https://doi.org/10.1007/978-1-61779-516-9_14" @default.
- W2258442600 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22237528" @default.
- W2258442600 hasPublicationYear "2011" @default.
- W2258442600 type Work @default.
- W2258442600 sameAs 2258442600 @default.
- W2258442600 citedByCount "4" @default.
- W2258442600 countsByYear W22584426002015 @default.
- W2258442600 countsByYear W22584426002016 @default.
- W2258442600 countsByYear W22584426002021 @default.
- W2258442600 crossrefType "book-chapter" @default.
- W2258442600 hasAuthorship W2258442600A5010332653 @default.
- W2258442600 hasAuthorship W2258442600A5050601584 @default.
- W2258442600 hasAuthorship W2258442600A5051083372 @default.
- W2258442600 hasConcept C104317684 @default.
- W2258442600 hasConcept C143425029 @default.
- W2258442600 hasConcept C185592680 @default.
- W2258442600 hasConcept C189338579 @default.
- W2258442600 hasConcept C24586158 @default.
- W2258442600 hasConcept C2908647359 @default.
- W2258442600 hasConcept C43617362 @default.
- W2258442600 hasConcept C4725764 @default.
- W2258442600 hasConcept C49105822 @default.
- W2258442600 hasConcept C54355233 @default.
- W2258442600 hasConcept C552990157 @default.
- W2258442600 hasConcept C71924100 @default.
- W2258442600 hasConcept C78063203 @default.
- W2258442600 hasConcept C86803240 @default.
- W2258442600 hasConcept C99454951 @default.
- W2258442600 hasConceptScore W2258442600C104317684 @default.
- W2258442600 hasConceptScore W2258442600C143425029 @default.
- W2258442600 hasConceptScore W2258442600C185592680 @default.
- W2258442600 hasConceptScore W2258442600C189338579 @default.
- W2258442600 hasConceptScore W2258442600C24586158 @default.
- W2258442600 hasConceptScore W2258442600C2908647359 @default.
- W2258442600 hasConceptScore W2258442600C43617362 @default.
- W2258442600 hasConceptScore W2258442600C4725764 @default.
- W2258442600 hasConceptScore W2258442600C49105822 @default.
- W2258442600 hasConceptScore W2258442600C54355233 @default.
- W2258442600 hasConceptScore W2258442600C552990157 @default.
- W2258442600 hasConceptScore W2258442600C71924100 @default.
- W2258442600 hasConceptScore W2258442600C78063203 @default.
- W2258442600 hasConceptScore W2258442600C86803240 @default.
- W2258442600 hasConceptScore W2258442600C99454951 @default.
- W2258442600 hasLocation W22584426001 @default.
- W2258442600 hasLocation W22584426002 @default.
- W2258442600 hasOpenAccess W2258442600 @default.
- W2258442600 hasPrimaryLocation W22584426001 @default.
- W2258442600 hasRelatedWork W1982638268 @default.
- W2258442600 hasRelatedWork W2032051433 @default.
- W2258442600 hasRelatedWork W2034029879 @default.
- W2258442600 hasRelatedWork W2146834312 @default.
- W2258442600 hasRelatedWork W2154911356 @default.
- W2258442600 hasRelatedWork W2258442600 @default.
- W2258442600 hasRelatedWork W2606140657 @default.
- W2258442600 hasRelatedWork W3008864600 @default.
- W2258442600 hasRelatedWork W3172927150 @default.
- W2258442600 hasRelatedWork W3204762792 @default.
- W2258442600 isParatext "false" @default.
- W2258442600 isRetracted "false" @default.
- W2258442600 magId "2258442600" @default.
- W2258442600 workType "book-chapter" @default.